Anti-Elafin/ESI antibody (ab192345)
Key features and details
- Rabbit polyclonal to Elafin/ESI
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Elafin/ESI antibody
See all Elafin/ESI primary antibodies -
Description
Rabbit polyclonal to Elafin/ESI -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Elafin/ESI aa 1 to the C-terminus.
Database link: P19957 -
Positive control
- WB: U-87 MG and HepG2 cell lysates. IHC-P: Human colon tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purity is > 95% (by SDS-PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
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IHC-P |
1/50 - 1/200.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa. |
IHC-P
1/50 - 1/200. |
Target
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Function
Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. -
Sequence similarities
Contains 1 WAP domain. -
Domain
Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5266 Human
- Omim: 182257 Human
- SwissProt: P19957 Human
- Unigene: 112341 Human
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Alternative names
- Cementoin antibody
- ELAF_HUMAN antibody
- Elafin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Elafin/ESI with ab192345 at 1/200 dilution.
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All lanes : Anti-Elafin/ESI antibody (ab192345)
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Predicted band size: 12 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab192345 has not yet been referenced specifically in any publications.