Anti-Elastase-1 antibody (ab231117)
Key features and details
- Rabbit polyclonal to Elastase-1
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Elastase-1 antibody
See all Elastase-1 primary antibodies -
Description
Rabbit polyclonal to Elastase-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment (His-tag) corresponding to Mouse Elastase-1 aa 1-300 (N terminal). (Expressed in E.coli).
Database link: Q91X79 -
Positive control
- WB: Recombinant mouse Elastase-1 protein, Mouse serum. ICH-P: Mouse testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa. |
Target
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Function
Acts upon elastin. -
Tissue specificity
Skin; not expressed in pancreas. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 109901 Mouse
- SwissProt: Q91X79 Mouse
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Alternative names
- CELA 1 antibody
- CELA1 antibody
- CELA1_HUMAN antibody
see all
Images
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DAB stained, formalin-fixed, paraffin-embedded mouse testis tissue stained for Elastase-1 using ab231117 at 20 µg/mL in immunohistochemical analysis.
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Anti-Elastase-1 antibody (ab231117) at 1 µg/ml + Mouse serum
Predicted band size: 29 kDa -
Anti-Elastase-1 antibody (ab231117) at 1 µg/ml + Recombinant mouse Elastase-1 protein.
Predicted band size: 29 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab231117 has been referenced in 1 publication.
- Tsang KM et al. Copper-Binding Domain Variation in a Novel Murine Lysyl Oxidase Model Produces Structurally Inferior Aortic Elastic Fibers Whose Failure Is Modified by Age, Sex, and Blood Pressure. Int J Mol Sci 23:N/A (2022). PubMed: 35743192