Overview

  • Product name
  • Description
    Rabbit polyclonal to Elastase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ELISA, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human, Pig
  • Immunogen

    Full length protein: Rat pancreatic elastase.

Properties

Applications

Our Abpromise guarantee covers the use of ab21593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
ELISA
WB
IP
ICC
  • Application notes
    ELISA: 1/1000 - 1/2000.
    ICC: 1/50 - 1/100.
    IHC-Fr: 1/1000 (PMID 20003319).
    IP: 1/100 - 1/200.
    WB: 1/200 - 1/500. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Efficient protease with alanine specificity but only little elastolytic activity.
    • Sequence similarities
      Belongs to the peptidase S1 family. Elastase subfamily.
      Contains 1 peptidase S1 domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • CEL3A_HUMAN antibody
      • CELA3A antibody
      • Chymotrypsin-like elastase family member 3A antibody
      • ELA1 antibody
      • ELA2A antibody
      • ELA2B antibody
      • ELA3 antibody
      • ELA3A antibody
      • ELA3B antibody
      • Elastase 1 antibody
      • Elastase 2A antibody
      • Elastase 2B antibody
      • Elastase 3A antibody
      • Elastase 3A pancreatic antibody
      • Elastase 3B antibody
      • Elastase 3B pancreatic antibody
      • Elastase IIIA antibody
      • Elastase IIIB antibody
      • Elastase-3A antibody
      • Fecal pancreatic elastase 1 antibody
      • HLE antibody
      • Medullasin antibody
      • Pancreatic elastase IIA antibody
      • PE 1 antibody
      • PE1 antibody
      • Protease E antibody
      see all

    Images

    • ab21593 staining Elastase in whole mouse embryos by Immunohistochemistry (PFA perfusion fixed frozen sections).
      Immunofluorescence was performed on e14.5, Nkx2.2-/- whole embryo frozen 8 µm sections that were fixed overnight with 4% paraformaldehyde. ab21593 was used at 1/1000. DAPI was used at 1/1000 and incubated for 30 minutes. Confocal images were taken on a Zeiss META LSM 510.
    • ab21593 staining Elastase in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/300 in BSA + TBS) for 30 minutes. An undiluted Goat anti-rabbit HRP polyclonal was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Hunter KE  et al. Identification and characterization of poorly differentiated invasive carcinomas in a mouse model of pancreatic neuroendocrine tumorigenesis. PLoS One 8:e64472 (2013). IHC-P ; Mouse . Read more (PubMed: 23691228) »
    • Holmstrom SR  et al. LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function. Genes Dev 25:1674-9 (2011). Read more (PubMed: 21852532) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Specification
    Pancreas
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
    Permeabilization
    No

    Abcam user community

    Verified customer

    Submitted Jun 11 2012

    Answer

    Thank you for your email.

    DISCOUNT CODE: xxx for ab9535 testing in dog
    DISCOUNT CODE: xxx for ab21593 testing in IHC-P
    DISCOUNT CODE: xxx for ab74868 testing in rat
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test these 3 antibodies in either dog, rat or IHC-P. Eachcode will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for either dog, rat or IHC-Pand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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