Product nameAnti-Elastase antibody
See all Elastase primary antibodies
DescriptionRabbit polyclonal to Elastase
Tested applicationsSuitable for: IHC-P, IHC-Fr, ELISA, WB, IP, ICCmore details
Species reactivityReacts with: Mouse
Does not react with: Human, Pig
Full length protein: Rat pancreatic elastase.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab21593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/50 - 1/100.
IHC-Fr: 1/1000 (PMID 20003319).
IP: 1/100 - 1/200.
WB: 1/200 - 1/500. Predicted molecular weight: 29 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionEfficient protease with alanine specificity but only little elastolytic activity.
Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain.
- Information by UniProt
- Entrez Gene: 67868 Mouse
- CEL3A_HUMAN antibody
- CELA3A antibody
- Chymotrypsin-like elastase family member 3A antibody
ab21593 staining Elastase in whole mouse embryos by Immunohistochemistry (PFA perfusion fixed frozen sections).
Immunofluorescence was performed on e14.5, Nkx2.2-/- whole embryo frozen 8 µm sections that were fixed overnight with 4% paraformaldehyde. ab21593 was used at 1/1000. DAPI was used at 1/1000 and incubated for 30 minutes. Confocal images were taken on a Zeiss META LSM 510.
ab21593 staining Elastase in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/300 in BSA + TBS) for 30 minutes. An undiluted Goat anti-rabbit HRP polyclonal was used as the secondary antibody.
This product has been referenced in:
- Hunter KE et al. Identification and characterization of poorly differentiated invasive carcinomas in a mouse model of pancreatic neuroendocrine tumorigenesis. PLoS One 8:e64472 (2013). IHC-P ; Mouse . Read more (PubMed: 23691228) »
- Holmstrom SR et al. LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function. Genes Dev 25:1674-9 (2011). Read more (PubMed: 21852532) »