Overview

  • Product name
  • Description
    Rabbit polyclonal to Elastin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, ICC/IF, IHC-P, ELISAmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Dog, Human, Pig
  • Immunogen

    Elastins pooled from pig, human, dog chicken, rat and cow.

Properties

Applications

Our Abpromise guarantee covers the use of ab21610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20138661
IHC-Fr Use at an assay dependent concentration. PubMed: 22884813
ICC/IF Use at an assay dependent concentration. PubMed: 22140132
IHC-P Use at an assay dependent concentration.
ELISA 1/1000 - 1/2000.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
    • Tissue specificity
      Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
    • Involvement in disease
      Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
      Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
      Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Sequence similarities
      Belongs to the elastin family.
    • Post-translational
      modifications
      Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
      Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
    • Information by UniProt
    • Database links
    • Alternative names
      • Elastin antibody
      • ELN antibody
      • ELN_HUMAN antibody
      • SVAS antibody
      • Tropoelastin antibody
      • WBS antibody
      • WS antibody
      see all

    Images

    • Immunohistochemical analysis of Bouin’s solution fixed (25cm H2O pressure for 90 min) paraffin-embedded wild type mouse lung (12mo, female) tissue labeling Elastin with ab21610 at 1/50 dilution, followed by HRP-conjugated secondary antibody.

      Left Panel: No antibody control.

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH-3T3 cells labeling Elastin with ab21610 at 1/200 dilution, followed by a FITC-conjugated secondary antibody. The nuclear counter stain is DAPI (blue).

    • Immunohistochemical analysis of frozen microfibrous vascular grafts from rat carotid artery tissue, staining Elastin with ab21610.

    References

    This product has been referenced in:
    • Fang CL  et al. Liposome-Encapsulated Baicalein Suppressed Lipogenesis and Extracellular Matrix Formation in Hs68 Human Dermal Fibroblasts. Front Pharmacol 9:155 (2018). Read more (PubMed: 29559910) »
    • Shirali AS  et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). Read more (PubMed: 29615716) »
    See all 25 Publications for this product

    Customer reviews and Q&As

    1-10 of 13 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Sheep Tissue sections (Carotid artery)
    Permeabilization
    Yes - 0.1% Triton-X
    Specification
    Carotid artery
    Blocking step
    Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde

    Ms. Chelsea Stowell

    Verified customer

    Submitted Jun 04 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Prostate)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Permeabilization
    No
    Specification
    Prostate
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 28 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab94674)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde

    Mr. David Ivancic

    Verified customer

    Submitted May 22 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Fibrotic Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
    Permeabilization
    No
    Specification
    Fibrotic Skin
    Blocking step
    Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
    Fixative
    10% NBF

    Premier Laboratory

    Verified customer

    Submitted Apr 05 2016

    Answer

    Thank you for contacting us and your interest in our products.
    The three antibodies you mentioned would all be expected to recognise the extracellular elastin. The mouse monoclonals ab9519 and ab97963 are of the same clone (BA-4) and has been shown to recognise insoluble elastin, alpha-elastin as well as the soluble non-cross linked precursor of elastin (tropoelastin).
    I hope this information has been of help. If you have any further questions, please do not hesitate to let us know.

    Read More

    Question
    Answer

    Thank you for your enquiry.

    I can confirm that ab21640 and ab9519 are sold as whole antiseruma and ascites respectively. Unpurified antibodies, such as these sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

    I can confirm that for whole antiserum, concentration of antibody is known to very between 1 - 10 mg/ml, while the Anti-Elastin antibody [BA-4] (ab9519) is estimated to be around 0.1-0.2 mg/mL.

    I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question
    Answer

    Thank you for contacting us.
    Your credit note ID is ****.
    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.
    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.
    The credit note ID is for your reference only and does not automatically guarantee the credit.
    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Answer

    Thank you for your message. I am sorry to hear you have previouslyhad difficultywith two of our antibodies.

    I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our 6 month Abpromise guarantee, which guarantees that the product will work in the tested applications and species specified on the individual datasheets, or we will offer a replacement, credit, or refund within 6 months of purchase. I can confirm that ab21610 has been tested and therefore is guarnateed for IHC-FoFr, ICC/IF, IHC-P, ELISA, WB on Mouse, Rat, Chicken, Cow, Dog, Human and Pig samples.

    For more details regarding our guarantee, please see the following page from our website:

    https://www.abcam.com/index.html?pageconfig=abpromise

    For the antibodies that you had problems with, I would like to reassure you that these will also be covered by the6 month guarantee for the applications and species on the datasheet. Have you contacted us about this previously? Webe pleased to investigate this further for you to provide assistance, and also obtain more information for our quality monitoring records. In order to proceed with this, I would appreciate if you could let me know which application you used, and I can send you the relevant questionnaire in order to obtain the required details. It will help you put the information we require together very easily.

    Thank you for your time and cooperation. I look forward to hearing back from you.

    Read More

    Question

    Storage conditions:at -20℃ 2) Description of the problem (high background, no band, wrong band size...) no band 3) Sample preparation: Name of the tissue/cell line: artery Sample species: mouse Type of sample : whole cell lysates Lysis buffer Beyotime (P0013) Protease inhibitors: Beyotime PMSF(100mM) Phosphatase inhibitors: No Reducing agent :No Boiling for ≥5 min? yes Protein loaded 10-20ug/lane Positive control:Yes. The band of β-actin is very good. Negative control :no 4) Percentage of gel 10% Type of membrane :PVDF Protein transfer verified: Yes(Fermentas protein ladders SM0671) Blocking agent and concentration: 5% MILK(5g in 100mlTBST) Blocking time 1—2 HOURS at room temperature or 4℃ for over night Blocking temperature 1—2 HOURS at room temperature or 4℃ for over night 5) Primary antibody (If more than one was used, describe in “additional notes”) Concentration or dilution: 0.3ug/ml Diluent buffer: PBS Incubation time : 4℃ for over night Incubation temperature: 4℃ for over night 6) Secondary antibody: IgG/HRP(ZDR-5307) Species:goat Reacts against: mouse Concentration or dilution :1:5000-1:10000 Diluent buffer : 5% MILK(5g in 100mlTBST) Incubation time: 1-2 hours Incubation temperature: room temperature Fluorochrome or enzyme conjugate: HRP 7) Washing after primary and secondary antibodies: Yse Buffer:1×TBST Number of washes: 3 time 8)Detection method : in dark room, used Kodak Scientific imaging film to reveal blot. 9) How many times have you run this staining? More than 20 time Do you obtain the same results every time? Yes What steps have you altered to try and optimize the use of this antibody? I used different dilution primary antibody from 1/200-1/500,but there were no band every time.

    Read More
    Answer

    Thank you for contacting Abcam.
    I am sorry about all of the issues you are having with ab21610 in western blotting. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse samples. Since I do not believe that there is any protocol advice that I could give that would help to resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.
    Please let me know how you would you like to continue and if you could also provide either the Abcam order number or the PO# used to purchase the antibody, that would be very helpful.

    Read More

    Question
    Answer

    Thank you for contacting Abcam regarding ab21610.


    I can confirm that this antibody will also react with tropoelastin.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Read More

    1-10 of 13 Abreviews or Q&A

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