- Datasheet
- Specific References (25)
- Protocols
Overview
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Product nameAnti-Elastin antibody
See all Elastin primary antibodies -
DescriptionRabbit polyclonal to Elastin
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, ICC/IF, IHC-P, ELISAmore details
Unsuitable for: WB -
Species reactivityReacts with: Mouse, Rat, Chicken, Dog, Human, Pig
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Immunogen
Elastins pooled from pig, human, dog chicken, rat and cow.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: None
Constituents: Whole serum. -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab21610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20138661 | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 22884813 | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 22140132 | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/1000 - 1/2000. |
Application notes
Is unsuitable for WB.
Target
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FunctionMajor structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
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Tissue specificityExpressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
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Involvement in diseaseDefects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
Sequence similaritiesBelongs to the elastin family.
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Post-translational
modificationsElastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. -
Cellular localizationSecreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
- Information by UniProt
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Database links
- Entrez Gene: 2006 Human
- Entrez Gene: 13717 Mouse
- Entrez Gene: 25043 Rat
- Omim: 130160 Human
- SwissProt: P15502 Human
- SwissProt: P54320 Mouse
- SwissProt: Q99372 Rat
- Unigene: 647061 Human
see all -
Alternative names
- Elastin antibody
- ELN antibody
- ELN_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody (ab21610)Immunohistochemical analysis of Bouin’s solution fixed (25cm H2O pressure for 90 min) paraffin-embedded wild type mouse lung (12mo, female) tissue labeling Elastin with ab21610 at 1/50 dilution, followed by HRP-conjugated secondary antibody.
Left Panel: No antibody control.
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Immunocytochemistry/ Immunofluorescence - Anti-Elastin antibody (ab21610)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH-3T3 cells labeling Elastin with ab21610 at 1/200 dilution, followed by a FITC-conjugated secondary antibody. The nuclear counter stain is DAPI (blue).
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Immunohistochemistry (Frozen sections) - Anti-Elastin antibody (ab21610)Image from Yu J et al., Biomaterials, Volume 33, Issue 32, November 2012, Pages 8062–8074. Fig 7.; doi: 10.1016/j.biomaterials.2012.07.042.Immunohistochemical analysis of frozen microfibrous vascular grafts from rat carotid artery tissue, staining Elastin with ab21610.
References
This product has been referenced in:
- Fang CL et al. Liposome-Encapsulated Baicalein Suppressed Lipogenesis and Extracellular Matrix Formation in Hs68 Human Dermal Fibroblasts. Front Pharmacol 9:155 (2018). Read more (PubMed: 29559910) »
- Shirali AS et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). Read more (PubMed: 29615716) »
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