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    elastin-antibody-ab21610.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Elastin
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Anti-Elastin antibody (ab21610)

  • Datasheet
  • SDS
Reviews (11)Q&A (9)References (50)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody (ab21610)
  • Immunocytochemistry - Anti-Elastin antibody (ab21610)

Key features and details

  • Rabbit polyclonal to Elastin
  • Suitable for: ICC, IHC-P
  • Reacts with: Mouse
  • Isotype: IgG

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Overview

  • Product name

    Anti-Elastin antibody
    See all Elastin primary antibodies
  • Description

    Rabbit polyclonal to Elastin
  • Host species

    Rabbit
  • Specificity

    Interacts with tropoelastin and insoluble elastin (there are many shared epitopes between the two forms of the protein).

  • Tested Applications & Species

    Application Species
    ICC
    Mouse
    IHC-P
    Mouse
    See all applications and species data
  • Immunogen

    Full length protein corresponding to Elastin. ab21610 was generated to the mature, insoluble form of elastin purified from several species.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Elastin
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Extracellular matrix

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab21610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Mouse
IHC-P
Mouse
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
IHC-P (6)
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
    • Tissue specificity

      Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
    • Involvement in disease

      Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
      Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
      Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Sequence similarities

      Belongs to the elastin family.
    • Post-translational
      modifications

      Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
      Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
    • Target information above from: UniProt accession P15502 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 13717 Mouse
      • SwissProt: P54320 Mouse
      • Unigene: 275320 Mouse
      • Unigene: 404771 Mouse
      • Alternative names

        • Elastin antibody
        • ELN antibody
        • ELN_HUMAN antibody
        • SVAS antibody
        • Tropoelastin antibody
        • WBS antibody
        • WS antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody (ab21610)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody (ab21610)

        Immunohistochemical analysis of Bouin’s solution fixed (25cm H2O pressure for 90 min) paraffin-embedded wild type mouse lung (12mo, female) tissue labeling Elastin with ab21610 at 1/50 dilution, followed by HRP-conjugated secondary antibody.

        Left Panel: No antibody control.

      • Immunocytochemistry - Anti-Elastin antibody (ab21610)
        Immunocytochemistry - Anti-Elastin antibody (ab21610)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH-3T3 cells labeling Elastin with ab21610 at 1/200 dilution, followed by a FITC-conjugated secondary antibody. The nuclear counter stain is DAPI (blue).

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (50)

      Publishing research using ab21610? Please let us know so that we can cite the reference in this datasheet.

      ab21610 has been referenced in 50 publications.

      • Ferreira JPS  et al. Altered mechanics of vaginal smooth muscle cells due to the lysyl oxidase-like1 knockout. Acta Biomater 110:175-187 (2020). PubMed: 32335309
      • Schappell LE  et al. A Microfluidic System to Measure Neonatal Lung Compliance Over Late Stage Development as a Functional Measure of Lung Tissue Mechanics. J Biomech Eng N/A:N/A (2020). PubMed: 32391560
      • Gremlich S  et al. Tenascin-C inactivation impacts lung structure and function beyond lung development. Sci Rep 10:5118 (2020). PubMed: 32198404
      • Niu YY  et al. Elevated intracellular copper contributes a unique role to kidney fibrosis by lysyl oxidase mediated matrix crosslinking. Cell Death Dis 11:211 (2020). PubMed: 32235836
      • Davidov T  et al. Porcine arterial ECM hydrogel: Designing an in vitro angiogenesis model for long-term high-throughput research. FASEB J 34:7745-7758 (2020). PubMed: 32337805
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-10 of 20 Abreviews or Q&A

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Elastin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Pig Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 (ab64236)
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 22 2020

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Elastin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 18 2020

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Elastin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Rat Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 12 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Elastin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Kidney H9 Organoids)
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney H9 Organoids
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 12 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Elastin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 14 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Elastin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 14 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Elastin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Pig Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 (ab64236)
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 14 2020

      Immunohistochemistry (Frozen sections) abreview for Anti-Elastin antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Sheep Tissue sections (Carotid artery)
      Permeabilization
      Yes - 0.1% Triton-X
      Specification
      Carotid artery
      Blocking step
      Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Fixative
      Paraformaldehyde
      Read More

      Dr. Chelsea Stowell

      Verified customer

      Submitted Jun 04 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Elastin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Prostate)
      Antigen retrieval step
      Enzymatic - Buffer/Enzyme Used: Proteinase K
      Permeabilization
      No
      Specification
      Prostate
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 28 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Elastin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab94674)
      Permeabilization
      Yes - 0.05% tween 20
      Specification
      Liver
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Mr. David Ivancic

      Verified customer

      Submitted May 22 2017

      1-10 of 20 Abreviews or Q&A

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