Anti-Elastin antibody (ab21610)

Thank you for contacting us and your interest in our products.
The three antibodies you mentioned would all be expected to recognise the extracellular elastin. The mouse monoclonals ab9519 and ab97963 are of the same clone (BA-4) and has been shown to recognise insoluble elastin, alpha-elastin as well as the soluble non-cross linked precursor of elastin (tropoelastin).
I hope this information has been of help. If you have any further questions, please do not hesitate to let us know.

Thank you for your enquiry.

I can confirm that ab21640 and ab9519 are sold as whole antiseruma and ascites respectively. Unpurified antibodies, such as these sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for whole antiserum, concentration of antibody is known to very between 1 - 10 mg/ml, while the Anti-Elastin antibody [BA-4] (ab9519) is estimated to be around 0.1-0.2 mg/mL.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Thank you for contacting us.
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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.
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The credit note ID is for your reference only and does not automatically guarantee the credit.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Thank you for your message. I am sorry to hear you have previouslyhad difficultywith two of our antibodies.

I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our 6 month Abpromise guarantee, which guarantees that the product will work in the tested applications and species specified on the individual datasheets, or we will offer a replacement, credit, or refund within 6 months of purchase. I can confirm that ab21610 has been tested and therefore is guarnateed for IHC-FoFr, ICC/IF, IHC-P, ELISA, WB on Mouse, Rat, Chicken, Cow, Dog, Human and Pig samples.

For more details regarding our guarantee, please see the following page from our website:

https://www.abcam.com/index.html?pageconfig=abpromise

For the antibodies that you had problems with, I would like to reassure you that these will also be covered by the6 month guarantee for the applications and species on the datasheet. Have you contacted us about this previously? Webe pleased to investigate this further for you to provide assistance, and also obtain more information for our quality monitoring records. In order to proceed with this, I would appreciate if you could let me know which application you used, and I can send you the relevant questionnaire in order to obtain the required details. It will help you put the information we require together very easily.

Thank you for your time and cooperation. I look forward to hearing back from you.

Thank you for contacting Abcam.
I am sorry about all of the issues you are having with ab21610 in western blotting. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse samples. Since I do not believe that there is any protocol advice that I could give that would help to resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.
Please let me know how you would you like to continue and if you could also provide either the Abcam order number or the PO# used to purchase the antibody, that would be very helpful.

Thank you for contacting Abcam regarding ab21610.


I can confirm that this antibody will also react with tropoelastin.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.