Key features and details
- Rabbit polyclonal to Elastin
- Suitable for: ICC, IHC-P
- Reacts with: Mouse, Rat, Chicken, Dog, Human, Pig
- Isotype: IgG
Product nameAnti-Elastin antibody
See all Elastin primary antibodies
DescriptionRabbit polyclonal to Elastin
Interacts with tropoelastin and insoluble elastin (there are many shared epitopes between the two forms of the protein).
Tested applicationsSuitable for: ICC, IHC-Pmore details
Unsuitable for: WB
Species reactivityReacts with: Mouse, Rat, Chicken, Dog, Human, Pig
Full length protein corresponding to Elastin. ab21610 was generated to the mature, insoluble form of elastin purified from several species.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab21610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
FunctionMajor structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
Tissue specificityExpressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
Involvement in diseaseDefects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Sequence similaritiesBelongs to the elastin family.
modificationsElastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Cellular localizationSecreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
- Information by UniProt
- Elastin antibody
- ELN antibody
- ELN_HUMAN antibody
Immunohistochemical analysis of Bouin’s solution fixed (25cm H2O pressure for 90 min) paraffin-embedded wild type mouse lung (12mo, female) tissue labeling Elastin with ab21610 at 1/50 dilution, followed by HRP-conjugated secondary antibody.
Left Panel: No antibody control.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH-3T3 cells labeling Elastin with ab21610 at 1/200 dilution, followed by a FITC-conjugated secondary antibody. The nuclear counter stain is DAPI (blue).
ab21610 has been referenced in 50 publications.
- Ferreira JPS et al. Altered mechanics of vaginal smooth muscle cells due to the lysyl oxidase-like1 knockout. Acta Biomater 110:175-187 (2020). PubMed: 32335309
- Schappell LE et al. A Microfluidic System to Measure Neonatal Lung Compliance Over Late Stage Development as a Functional Measure of Lung Tissue Mechanics. J Biomech Eng N/A:N/A (2020). PubMed: 32391560
- Gremlich S et al. Tenascin-C inactivation impacts lung structure and function beyond lung development. Sci Rep 10:5118 (2020). PubMed: 32198404
- Niu YY et al. Elevated intracellular copper contributes a unique role to kidney fibrosis by lysyl oxidase mediated matrix crosslinking. Cell Death Dis 11:211 (2020). PubMed: 32235836
- Davidov T et al. Porcine arterial ECM hydrogel: Designing an in vitro angiogenesis model for long-term high-throughput research. FASEB J 34:7745-7758 (2020). PubMed: 32337805