Overview

  • Product name

  • Description

    Rabbit polyclonal to Elastin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Elastin aa 750-786 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    GAGQFPLGGVA ARPGFGLSPI FPGGACLGKA CGRKRK


    Database link: P15502-3

  • Positive control

    • IHC-P: Human artery and glioma tissues; rat intervertebral disk tissue; mouse heart tissues. WB: Mouse lung and intestine lysates, rat lung lysates. FCM: A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab217356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/500.
Flow Cyt 1/50.

Target

  • Function

    Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
  • Tissue specificity

    Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
  • Involvement in disease

    Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
    Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
    Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similarities

    Belongs to the elastin family.
  • Post-translational
    modifications

    Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
  • Information by UniProt
  • Database links

  • Alternative names

    • Elastin antibody
    • ELN antibody
    • ELN_HUMAN antibody
    • SVAS antibody
    • Tropoelastin antibody
    • WBS antibody
    • WS antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human artery tissue labeling Elastin with ab217356 at 1/100 dilution followed by conjugation to the secondary antibody and DAB staining.

  • All lanes : Anti-Elastin antibody (ab217356) at 1/200 dilution

    Lane 1 : mouse intestine lysate
    Lane 2 : rat lung lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

    Predicted band size: 68 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human glioma tissue labeling Elastin with ab217356 at 1/100 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat intervertebral disk tissue labeling Elastin with ab217356 at 1/100 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue labeling Elastin with ab217356 at 1/1000 dilution followed by conjugation to the secondary antibody.

  • Flow cytometric analysis of A549 cells labeling Elastin with ab217356 at 1/50 dilution for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1/100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

  • Anti-Elastin antibody (ab217356) at 1/300 dilution + mouse lung lysate

    Secondary
    conjugated secondary at 1/10000 dilution

    Predicted band size: 68 kDa

References

This product has been referenced in:

  • Sun YY  et al. Sickle Mice Are Sensitive to Hypoxia/Ischemia-Induced Stroke but Respond to Tissue-Type Plasminogen Activator Treatment. Stroke 48:3347-3355 (2017). Read more (PubMed: 29127268) »
See 1 Publication for this product

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