Key features and details
- Rabbit polyclonal to Elastin
- Suitable for: ICC/IF, ELISA, RIA, IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Elastin antibody
See all Elastin primary antibodies
DescriptionRabbit polyclonal to Elastin
Specificityab23748 reacts with rat elastin 100%, rat collagen type I, II, III and V <0.1%
Tested applicationsSuitable for: ICC/IF, ELISA, RIA, IHC-Pmore details
Species reactivityReacts with: Rat
Purified elastin from rat aorta.
- Rat skin or aorta
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab23748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/600. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
FunctionMajor structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
Tissue specificityExpressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
Involvement in diseaseDefects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Sequence similaritiesBelongs to the elastin family.
modificationsElastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Cellular localizationSecreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
- Information by UniProt
- Elastin antibody
- ELN antibody
- ELN_HUMAN antibody
ab23748 has been referenced in 2 publications.
- Casco M et al. Iron Oxide Nanoparticles Stimulates Extra-Cellular Matrix Production in Cellular Spheroids. Bioengineering (Basel) 4:N/A (2017). PubMed: 28952483
- Olsen TR et al. Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces. Bioengineering (Basel) 3:N/A (2016). PubMed: 28952591