Anti-Elastin antibody [BA-4] (ab9519)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [BA-4] (ab9519)](https://www.abcam.com/ps/products/9/ab9519/Images/ab9519-320165-Ab9519AP4186837Huduodenum1ugml.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [BA-4] (ab9519)")
Key features and details
- Mouse monoclonal [BA-4] to Elastin
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Sheep, Goat, Guinea pig, Cat, Dog, Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Elastin antibody [BA-4]
See all Elastin primary antibodies -
Description
Mouse monoclonal [BA-4] to Elastin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Sheep, Goat, Guinea pig, Cat, Dog, Human, Pig -
Immunogen
corresponding to Bovine Elastin. Bovine alpha-Elastin.
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Epitope
BA-4 is specific for a chemotactically active epitope composed of valine, glycine, alanine, and proline in a molar ratio of approximately 2:2:1:1 (PMID 2429696). -
Positive control
- WB: Human skin tissue lysate. IHC-P: FFPE normal Human Duodenum tissue sections IHC-Fr: Human normal skin
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General notes
Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues. -
Clonality
Monoclonal -
Clone number
BA-4 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab9519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.51 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use a concentration of 1 µg/ml. |
Target
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Function
Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. -
Tissue specificity
Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin. -
Involvement in disease
Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
Sequence similarities
Belongs to the elastin family. -
Post-translational
modificationsElastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers. - Information by UniProt
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Database links
- Entrez Gene: 2006 Human
- Omim: 130160 Human
- SwissProt: P15502 Human
- Unigene: 647061 Human
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Alternative names
- Elastin antibody
- ELN antibody
- ELN_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [BA-4] (ab9519)
IHC image of Elastin staining in a section of formalin-fixed paraffin-embedded normal human normal duodenum* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab9519, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [BA-4] (ab9519)](https://www.abcam.com/ps/products/9/ab9519/Images/ab9519-316574-IHCAP418683701.jpg)
Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [BA-4] (ab9519)IHC image of ab9519 staining in 10% formaldehyde fixed frozen tissue section of normal human skin.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab9519 (1μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab9519.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [BA-4] (ab9519)](https://www.abcam.com/ps/products/9/ab9519/Images/ab9519-17219-anti-elastin-antibody-ba-4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [BA-4] (ab9519)This image is courtesy of an anonymous Abreviewab9519 staining pig small intestine tissue sections by IHC-P. Sections were fixed with Bouins and subjected to an enzymatic antigen retreival step. The primary antibody was diluted 1/200 in a commercially available antibody diluent (replacing the blocking step) and incubated with the sample for 1 hour at 20°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.
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![Western blot - Anti-Elastin antibody [BA-4] (ab9519)](https://www.abcam.com/ps/products/9/ab9519/Images/ab9519-17218-anti-elastin-antibody-ba-4-western-blot.jpg)
Western blot - Anti-Elastin antibody [BA-4] (ab9519)Anti-Elastin antibody [BA-4] (ab9519) at 0.51 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Protocols
References (14)
ab9519 has been referenced in 14 publications.
- Fazaeli S et al. Alteration of structural and mechanical properties of the temporomandibular joint disc following elastase digestion. J Biomed Mater Res B Appl Biomater N/A:N/A (2020). PubMed: 32478918
- Campo H et al. De- and recellularization of the pig uterus: a bioengineering pilot study. Biol Reprod 96:34-45 (2017). PubMed: 28395322
- Godinho MSC et al. Elastin is Localised to the Interfascicular Matrix of Energy Storing Tendons and Becomes Increasingly Disorganised With Ageing. Sci Rep 7:9713 (2017). PubMed: 28855560
- Kawamoto-Hirano A et al. Cricoarytenoid Articulation in Elderly Japanese With Special Reference to Morphology of the Synovial Tissue. Ann Otol Rhinol Laryngol 125:219-27 (2016). PubMed: 26391093
- Yang YF et al. Effects of induction and inhibition of matrix cross-linking on remodeling of the aqueous outflow resistance by ocular trabecular meshwork cells. Sci Rep 6:30505 (2016). WB ; Human . PubMed: 27465745
