Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Elastin antibody [BA-4]

Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lung Artery)
Specification
Lung Artery
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Prot K
Permeabilization
No

Other product details

Dilution
1/25
Incubation time
1 hour(s) and 0 minute(s) · Diluent: BSA in TBS

Secondary antibody

Name
Non-Abcam antibody was used: RbaMs IgG then Envision Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
The antibody showed no staining in any elastic fibers in several rat tissues. The optimized protocol for human tissue also showed no staining in rat tissue. Heat retrievals were also tested with negative results. This antibody seems to not be reactive with rat elastin.
Abcam response
Thank you for your honest opinion, even if the results are negative. We encourage our customers to send us their results as this is valuable information.

However, the antibody has not been tested in the species, so further testing results with rats are very welcome.

Abcam user community

Verified customer

Submitted Jul 11 2012

Sign up