Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Elastin antibody [BA-4]

Below Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: donkey anti-mouse IgG-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Abcam response
Our Scientific Support team has investigated this negative Abreview, and we have resolved the issue in accordance with our Abpromise.

Abcam user community

Verified customer

Submitted Jun 10 2016

Sign up