Key features and details
- Rabbit polyclonal to Elav-type RNA-binding protein ETR3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Elav-type RNA-binding protein ETR3 antibody
See all Elav-type RNA-binding protein ETR3 primary antibodies
DescriptionRabbit polyclonal to Elav-type RNA-binding protein ETR3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Xenopus laevis, Zebrafish
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 198 of Human Elav-type RNA-binding protein ETR3 (UniProt ID: O95319).
- NT2D1 whole cell lysate; IMR32 lysate; MCF7 cells
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab111728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 54 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionRNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats.
Tissue specificityExpressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the CELF/BRUNOL family.
Contains 3 RRM (RNA recognition motif) domains.
Developmental stageIsoform 1 is expressed in fetal brain. Isoform 2 is expressed in fetal heart, brain, thymus, lung, liver, skeletal muscle, kidney and spleen. Isoform 4 is expressed in fetal heart, brain, thymus, lung and skeletal muscle.
Cellular localizationNucleus. Cytoplasm. Accumulates in the cytoplasm after ionizing radiation (By similarity). Colocalizes with APOBEC1 and A1CF. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
- Information by UniProt
- Bruno-like protein 3 antibody
- BRUNOL3 antibody
- Cardiac elav type RNA binding protein antibody
Anti-Elav-type RNA-binding protein ETR3 antibody (ab111728) at 1/3000 dilution + NT2D1 whole cell lysate
at 30 µg
Predicted band size: 54 kDa
7.5% SDS PAGE
Immunofluorescence analysis of Elav-type RNA-binding protein ETR3 in paraformaldehyde-fixed MCF7, using ab111728 at a 1/500 dilution. Lower image shows cells counter stained with Hoechst 33342.
ab111728 has been referenced in 1 publication.
- Blech-Hermoni Y et al. Diversity and conservation of CELF1 and CELF2 RNA and protein expression patterns during embryonic development. Dev Dyn 242:767-77 (2013). PubMed: 23468433