Recombinant
RabMAb

Recombinant Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

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Overview

  • Product name

    Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2]
    See all Elav-type RNA-binding protein ETR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13374-2] to Elav-type RNA-binding protein ETR3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Elav-type RNA-binding protein ETR3 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: O95319

  • Positive control

    • Human fetal liver, K562, HeLa, MCF7, C6 and NIH 3T3 lysates; Human brain tissue; rat lung tissue; MCF7 and HeLa cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.
Flow Cyt 1/270.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP 1/50 - 1/70.

Target

  • Function

    RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats.

  • Tissue specificity

    Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.

  • Sequence similarities

    Belongs to the CELF/BRUNOL family.
    Contains 3 RRM (RNA recognition motif) domains.

  • Developmental stage

    Isoform 1 is expressed in fetal brain. Isoform 2 is expressed in fetal heart, brain, thymus, lung, liver, skeletal muscle, kidney and spleen. Isoform 4 is expressed in fetal heart, brain, thymus, lung and skeletal muscle.

  • Cellular localization

    Nucleus. Cytoplasm. Accumulates in the cytoplasm after ionizing radiation (By similarity). Colocalizes with APOBEC1 and A1CF. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Bruno-like protein 3 antibody
    • BRUNOL3 antibody
    • Cardiac elav type RNA binding protein antibody
    • CELF-2 antibody
    • celf2 antibody
    • CELF2_HUMAN antibody
    • CUG BP2 antibody
    • CUG triplet repeat RNA-binding protein 2 antibody
    • CUG-BP- and ETR-3-like factor 2 antibody
    • CUG-BP2 antibody
    • CUGBP Elav like family member 2 antibody
    • CUGBP Elav-like family member 2 antibody
    • CUGBP2 antibody
    • dJ323N1.1 antibody
    • elav type RNA binding protein antibody
    • ELAV-type RNA-binding protein 3 antibody
    • ETR 3 antibody
    • ETR-3 antibody
    • ETR3 antibody
    • HGNC:2550 antibody
    • hNAPOR antibody
    • NAPOR 2 antibody
    • NAPOR antibody
    • Neuroblastoma apoptosis-related RNA-binding protein antibody
    • Neuroplastoma apoptosis related RNA binding protein 3 antibody
    • RNA binding protein BRUNOL3 antibody
    • RNA-binding protein BRUNOL-3 antibody
    see all

Images

  • Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    All lanes : Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    ab186430 staining Elav-type RNA-binding protein ETR3 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1:PBS only.

  • Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    All lanes : Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Flow Cytometry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Flow Cytometry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/270 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Western blot analysis of Elav-type RNA-binding protein ETR3 in HeLa cell lysate immunoprecipitated with ab186430 at 1/50 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

This product has been referenced in:

  • Lukosiute-Urboniene A  et al. Human antigen R mediated post-transcriptional regulation of inhibitors of apoptosis proteins in pancreatic cancer. World J Gastroenterol 25:205-219 (2019). Read more (PubMed: 30670910) »
  • Yeung YT  et al. CELF2 suppresses non-small cell lung carcinoma growth by inhibiting the PREX2-PTEN interaction. Carcinogenesis N/A:N/A (2019). Read more (PubMed: 31241130) »
See all 3 Publications for this product

Publishing research using ab186430? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Permeabilization
Yes - 0.3% Triton X-100 in blocking buffer
Specification
fibroblast
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 12 2018

Western blot abreview for Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris, MES Buffer)
Loading amount
14 µg
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 24 2017

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