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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing
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RecombinantRabMAb

Recombinant Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)

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Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)
  • Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)
  • Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13374] to Elav-type RNA-binding protein ETR3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374]
    See all Elav-type RNA-binding protein ETR3 primary antibodies
  • Description

    Rabbit monoclonal [EPR13374] to Elav-type RNA-binding protein ETR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Elav-type RNA-binding protein ETR3. The exact sequence is proprietary.
    Database link: O95319

  • Positive control

    • Fetal liver, MCF7, K562 and HeLa lysates; Human pancreas tissue; MCF7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13374
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Alternative Versions

    • Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] - BSA and Azide free (ab250091)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats.
    • Tissue specificity

      Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.
    • Sequence similarities

      Belongs to the CELF/BRUNOL family.
      Contains 3 RRM (RNA recognition motif) domains.
    • Developmental stage

      Isoform 1 is expressed in fetal brain. Isoform 2 is expressed in fetal heart, brain, thymus, lung, liver, skeletal muscle, kidney and spleen. Isoform 4 is expressed in fetal heart, brain, thymus, lung and skeletal muscle.
    • Cellular localization

      Nucleus. Cytoplasm. Accumulates in the cytoplasm after ionizing radiation (By similarity). Colocalizes with APOBEC1 and A1CF. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
    • Target information above from: UniProt accession O95319 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10659 Human
      • Entrez Gene: 14007 Mouse
      • Entrez Gene: 29428 Rat
      • Omim: 602538 Human
      • SwissProt: O95319 Human
      • SwissProt: Q9Z0H4 Mouse
      • SwissProt: Q792H5 Rat
      • Unigene: 309288 Human
      • Unigene: 398543 Mouse
      • Unigene: 162247 Rat
      see all
    • Alternative names

      • Bruno-like protein 3 antibody
      • BRUNOL3 antibody
      • Cardiac elav type RNA binding protein antibody
      • CELF-2 antibody
      • celf2 antibody
      • CELF2_HUMAN antibody
      • CUG BP2 antibody
      • CUG triplet repeat RNA-binding protein 2 antibody
      • CUG-BP- and ETR-3-like factor 2 antibody
      • CUG-BP2 antibody
      • CUGBP Elav like family member 2 antibody
      • CUGBP Elav-like family member 2 antibody
      • CUGBP2 antibody
      • dJ323N1.1 antibody
      • elav type RNA binding protein antibody
      • ELAV-type RNA-binding protein 3 antibody
      • ETR 3 antibody
      • ETR-3 antibody
      • ETR3 antibody
      • HGNC:2550 antibody
      • hNAPOR antibody
      • NAPOR 2 antibody
      • NAPOR antibody
      • Neuroblastoma apoptosis-related RNA-binding protein antibody
      • Neuroplastoma apoptosis related RNA binding protein 3 antibody
      • RNA binding protein BRUNOL3 antibody
      • RNA-binding protein BRUNOL-3 antibody
      see all

    Images

    • Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)
      Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)
      All lanes : Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : MCF7 lysate
      Lane 3 : K562 lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 54 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)
      Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)

      Immunofluorescent analysis of MCF7 cells labeling Elav-type RNA-binding protein ETR3 with ab179447 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Elav-type RNA-binding protein ETR3 with ab179447 at 1/50 dilution.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)
      Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (ab179447)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab179447? Please let us know so that we can cite the reference in this datasheet.

    ab179447 has not yet been referenced specifically in any publications.

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