Product nameAnti-ELAVL2 + ELAVL4 antibody
DescriptionRabbit polyclonal to ELAVL2 + ELAVL4
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from an internal region of human ELAVL2 + ELAVL4.
- Extracts from HUVEC cells and Jurkat cells. HepG2 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weights: 40 and 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceELAV2 binds RNA and seems to recognize a GAAA motif and is neuronal specific. ELAVL4 may play a role in neuron-specific RNA processing. It contains 3 RNA recognition motif (RRM) domains and is localised to brain tissue. ELAVL4 has been linked with the disease, paraneoplastic encephalomyelitis sensory neuropathy, which is associated with small cell lung cancer and is characterized by dementia, sensory loss, and other neurological disabilities. This disease may result from an immune response primarily directed against a small cell lung tumor antigen which is misdirected against similar antigens expressed in brain (such as ELAVL4).
- ELAV like 4 antibody
- ELAV like neuronal protein 1 antibody
- ELAV like protein 2 antibody
All lanes : Anti-ELAVL2 + ELAVL4 antibody (ab72603) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from Jurkat cells
Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 40 , 42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa, 80 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
ab72603, at 1/500 dilution, staining HepG2 cells in the absence or presence of the immunising peptide.
ab72603 has not yet been referenced specifically in any publications.