Overview

  • Product name

    Anti-ELAVL2 + ELAVL4 antibody
  • Description

    Rabbit polyclonal to ELAVL2 + ELAVL4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human ELAVL2 + ELAVL4.

  • Positive control

    • Extracts from HUVEC cells and Jurkat cells. HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notes
    ELISA: 1/10000.
    ICC/IF: 1/500 - 1/1000.
    WB: 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weights: 40 and 42 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      ELAV2 binds RNA and seems to recognize a GAAA motif and is neuronal specific. ELAVL4 may play a role in neuron-specific RNA processing. It contains 3 RNA recognition motif (RRM) domains and is localised to brain tissue. ELAVL4 has been linked with the disease, paraneoplastic encephalomyelitis sensory neuropathy, which is associated with small cell lung cancer and is characterized by dementia, sensory loss, and other neurological disabilities. This disease may result from an immune response primarily directed against a small cell lung tumor antigen which is misdirected against similar antigens expressed in brain (such as ELAVL4).
    • Database links

    • Alternative names

      • ELAV like 4 antibody
      • ELAV like neuronal protein 1 antibody
      • ELAV like protein 2 antibody
      • ELAV like protein 4 antibody
      • ELAVL 2 antibody
      • ELAVL 4 antibody
      • HELN1 antibody
      • Hu antigen B antibody
      • Hu antigen D antibody
      • HUB antibody
      • HUD antibody
      see all

    Images

    • All lanes : Anti-ELAVL2 + ELAVL4 antibody (ab72603) at 1/500 dilution

      Lane 1 : Extracts from HUVEC cells
      Lane 2 : Extracts from Jurkat cells
      Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 40 , 42 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 75 kDa, 80 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

    • ab72603, at 1/500 dilution, staining HepG2 cells in the absence or presence of the immunising peptide.

    References

    ab72603 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (NPCs derived from iPSC)
    Permeabilization
    Yes - 0.25% Triton X-100 in PBS
    Specification
    NPCs derived from iPSC
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 10 2016

    Answer

    Thank you for your reply and sorry for the delay in mine.



    I have aligned our exact immunogen sequence for ab136069 with the provided protein sequences. The homology score is listed below:

    Dogfish Elavl4: 92.9% identity in a 14 residue overlap; Score 73.0; Gap frequency: 0.0%
    Brafl Elav: 78.6% identity in 14 residue overlap; Score 62.0; Gap frequency: 0.0%
    Pomla Elav: 58.3% identity in 12 residue overlap; Score 40.0; Gap frequency: 0.0%
    Pladu Elav: 78.6% identity in 14 residue overlap; Score 55.0; Gap frequency: 0.0%
    Patvu Elav: 71.4% identity in 14 residue overlap; Score 43.0; Gap frequency: 0.0%
    Human Elavl2 50-100 immunogen: 100% identity in 14 residue overlap; Score 74.0; Gap frequency: 0.0%

    We would generally say that a homology of 85% and above are worth trying to see if there is cross reactivity. As we have not tested the antibody in any of these species we would not be able to guarentee its cross reactivity. However, if you would like to try the antibody with your dogfish samples you would be eligable to take part in our testing discount scheme.
    This offer means that if you purchase ab136069 as normal, test the antibody with your dogfish samples and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab136069 (£227) off any product in our catalogue. More information on this offer can be found here:

    www.abcam.com/abtrial
    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.
    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab13606I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

    Read More

    Answer

    I can now share some further information with you in regards to the other anti-ELAVL2 + ELAVL4 antibody we have on our catalogue, ab136069.

    The immunogen used to raise this antibody has been taken from within the residues 50-100 of human ELAV2/4 with accession number Q12926 (http://www.uniprot.org/uniprot/Q12926).

    This antibody binds to the RRM1 domain of isoforms 1 and 2.

    I hope this may have higher homology with your target. Although if we have not tested an antibody in the species you are hoping to use the antibody with, we would not be able to guarantee its performance. However, we do have a testing discount code scheme which may be of interest to you. More information on this scheme can be found from the following link:

    www.abcam.com/abtrial

    Please bear in mind we need to raise a discount code prior to you purchasing the antibody you would be interested in testing so please do let me know if you would like to participate in the scheme.

    I hope this has been of some help. If I can be of any further assistance, please do not hesitate to let me know.

    Until then, I wish you all the best with your research.

    Read More

    Answer

    Many thanks for contacting us and your interest in our products.



    The exact immunogen used to raise the Anti-ELAVL2 + ELAVL4 antibody (ab72603) is proprietary information. However, I am able to share with you that the immunogen used to raise this antibody is a synthetic peptide taken from the region 10-60 of human ELAVL2 with accession number Q12926 (http://www.uniprot.org/uniprot/Q12926).



    This antibody binds to isoform 1 and 2. If you would not mind sharing the sequences of the proteins you are interested I can check for the homology shared between these proteins and the immunogen in order to predict the cross reactivity.



    I hope this information has been of some help. If you have any further questions, please do not hesitate to ask.

    Read More

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