Anti-ELF1 antibody (ab64937)
Key features and details
- Rabbit polyclonal to ELF1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ELF1 antibody
See all ELF1 primary antibodies -
Description
Rabbit polyclonal to ELF1 -
Host species
Rabbit -
Specificity
ab64937 detects endogenous levels of total ELF1 protein.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ELF1 (C terminal).
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Positive control
- A549 cells and cell extracts from 293 and HuvEc cells.
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General notes
This product was previously labelled as E74 like factor 1
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab64937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500 - 1/1000. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. -
Tissue specificity
In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1997 Human
- Omim: 189973 Human
- SwissProt: P32519 Human
- Unigene: 135646 Human
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Alternative names
- E74 like factor 1 antibody
- E74-like factor 1 antibody
- ELF 1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ELF1 antibody (ab64937)This image shows A549 cells stained with ab64937 at 1/500 dilution; with and without the immunising peptide.
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Western blot - Anti-ELF1 antibody (ab64937)All lanes : Anti-ELF1 antibody (ab64937) at 1/500 dilution
Lane 1 : extracts from 293 cells (5-30µg total protein)
Lane 2 : extracts from HuvEc cells (5-30µg total protein)
Lane 3 : extracts from HuvEc cells (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Protocols
Datasheets and documents
References (1)
ab64937 has been referenced in 1 publication.
- Ben Khalaf N et al. The mouse intron-nested gene, Israa, is expressed in the lymphoid organs and involved in T-cell activation and signaling. Mol Immunol 111:209-219 (2019). PubMed: 31096062
