Anti-ELF1 antibody (ab64937)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ELF1 antibody
See all ELF1 primary antibodies -
Description
Rabbit polyclonal to ELF1 -
Host species
Rabbit -
Specificity
ab64937 detects endogenous levels of total ELF1 protein.
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Tested applications
Suitable for: ELISA, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ELF1 (C terminal).
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Positive control
- A549 cells and cell extracts from 293 and HuvEc cells.
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General notes
This product was previously labelled as E74 like factor 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab64937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/5000. | |
| ICC/IF | 1/500 - 1/1000. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. -
Tissue specificity
In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1997 Human
- Omim: 189973 Human
- SwissProt: P32519 Human
- Unigene: 135646 Human
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Alternative names
- E74 like factor 1 antibody
- E74-like factor 1 antibody
- ELF 1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ELF1 antibody (ab64937)This image shows A549 cells stained with ab64937 at 1/500 dilution; with and without the immunising peptide. -
Western blot - Anti-ELF1 antibody (ab64937)All lanes : Anti-ELF1 antibody (ab64937) at 1/500 dilution
Lane 1 : extracts from 293 cells (5-30µg total protein)
Lane 2 : extracts from HuvEc cells (5-30µg total protein)
Lane 3 : extracts from HuvEc cells (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Datasheets and documents
References
ab64937 has not yet been referenced specifically in any publications.
