Key features and details
- Rabbit polyclonal to ELF1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ELF1 antibody
See all ELF1 primary antibodies
DescriptionRabbit polyclonal to ELF1
ab64937 detects endogenous levels of total ELF1 protein.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ELF1 (C terminal).
- A549 cells and cell extracts from 293 and HuvEc cells.
This product was previously labelled as E74 like factor 1
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|WB||1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).|
FunctionTranscription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.
Tissue specificityIn fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- E74 like factor 1 antibody
- E74-like factor 1 antibody
- ELF 1 antibody
This image shows A549 cells stained with ab64937 at 1/500 dilution; with and without the immunising peptide.
All lanes : Anti-ELF1 antibody (ab64937) at 1/500 dilution
Lane 1 : extracts from 293 cells (5-30µg total protein)
Lane 2 : extracts from HuvEc cells (5-30µg total protein)
Lane 3 : extracts from HuvEc cells (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size: 67 kDa
Observed band size: 67 kDa
ab64937 has been referenced in 1 publication.
- Ben Khalaf N et al. The mouse intron-nested gene, Israa, is expressed in the lymphoid organs and involved in T-cell activation and signaling. Mol Immunol 111:209-219 (2019). PubMed: 31096062