Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593)

Overview

  • Product name

    Anti-elF2 alpha (phospho S52) antibody - N-terminal
  • Description

    Rabbit polyclonal to elF2 alpha (phospho S52) - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human elF2 alpha (N terminal) (phospho S52). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: P05198

  • Positive control

    • WB: HEK-293T whole cell extract treated with/without anisomycin; PC-12 and HepG2 whole cell extracts treated with/without thapsigargin; HeLa whole cell extract treated with/without tunicamycin.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab227593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.

Target

  • Database links

  • Alternative names

    • eIF-2-alpha antibody
    • eIF-2A antibody
    • eIF-2alpha antibody
    • Eukaryotic translation initiation factor 2 subunit 1 antibody
    • Eukaryotic translation initiation factor 2 subunit alpha antibody
    see all

Images

  • All lanes : Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 4 µg/ml tunicamycin for 16 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 3 µM thapsigargin for 12 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) treated with 300 µM thapsigargin for 8 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) treated with 25 µg/ml anisomycin for 16 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS-PAGE gel.

References

ab227593 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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