Key features and details
- Rabbit polyclonal to ELF2/NERF
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ELF2/NERF antibody
DescriptionRabbit polyclonal to ELF2/NERF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ELF2/NERF aa 1-140.
Database link: Q15723
- WB: HL-60, A549, MCF7, Jurkat and HeLa whole cell lysate. IHC-P: Human skin and placenta tissue.
This product was previously labelled as ELF2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|IHC-P||1/20 - 1/200.|
RelevanceThe ELF2 gene encodes a protein that physically interacts with AML1 and mediates opposing effects on AML1 mediated transcription of the B cell specific blk gene.
- SwissProt: Q15723 Human
- E74 like factor 2 antibody
- E74 like factor 2 ets domain transcription factor antibody
- ELF 2 antibody
All lanes : Anti-ELF2/NERF antibody (ab225958) at 1/1000 dilution
Lane 1 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Developed using the ECL technique.
Paraffin embedded human skin tissue stained for ELF2/NERF with ab225958 (1/100 dilution) in immunohistochemical analysis.
Paraffin embedded human placenta tissue stained for ELF2/NERF with ab225958 (1/100 dilution) in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225958 has not yet been referenced specifically in any publications.