• Product name
  • Description
    Rabbit polyclonal to Elf4/MEF
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Elf4/MEF.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse testis; Mouse lung; Mouse spleen as well as the following whole cell lysates: NIH3T3; K562.



Our Abpromise guarantee covers the use of ab96075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa).


  • Function
    Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T cells via the Kruppel-like factors KLF4 and KLF2 (By similarity). Controls cell senescence in a p53-dependent manner. Can also promote celular transformation through inhibition of the p16 pathway.
  • Tissue specificity
    Abundantly expressed in the placenta and in a variety of myeloid leukemia cell lines. Moderate levels of expression in heart, lung, spleen, thymus, peripheral blood lymphocytes, ovary and colon. Lower levels of expression in Jurkat T-cells and other T-cell lines and no expression in brain.
  • Involvement in disease
    A chromosomal aberration involving ELF4 is a cause of acute myeloid leukemia (AML). Translocation t(X;21)(q25-26;q22) with ERG.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Cellular localization
    Nucleus > PML body. Accumulation into PML nuclear bodies is mediated by PML.
  • Information by UniProt
  • Database links
  • Alternative names
    • E74-like factor 4 antibody
    • ELF4 antibody
    • ELF4_HUMAN antibody
    • ELFR antibody
    • ETS-related transcription factor Elf-4 antibody
    • ETS-related transcription factor Elf4 antibody
    • MEF antibody
    • Myeloid Elf-1-like factor antibody
    • Myeloid Elf1-like factor antibody
    see all


  • All lanes : Anti-Elf4/MEF antibody (ab96075) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 3 : Spleen (Mouse) Tissue Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 4 minutes


ab96075 has not yet been referenced specifically in any publications.

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