Key features and details
- Goat polyclonal to ELF5
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ELF5 antibody
See all ELF5 primary antibodies
DescriptionGoat polyclonal to ELF5
SpecificityThis antibody is expected to recognize both reported isoforms (as represented by NP_001413; NP_938195)
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
General notesGenBank Accession Number - NP_001413; NP_938195
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab13581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionTranscriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation.
Isoform 2 binds to DNA sequences containing the consensus nucleotide core sequence GGA[AT]. Transcriptionally activates SPRR2A and the parotid gland-specific PSP promoters.
Tissue specificityExpressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, kidney and prostate. Weakly expressed in placenta and lung. Isoform 1 and isoform 2 are differentially expressed in different tissues. In the kidney, only isoform 1 was expressed, while prostate expressed both isoforms, with levels of isoform 2 being higher. Expression is up-regulated during keratinocyte differentiation. Several epithelial carcinoma cell lines showed lack of expression.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
DomainThe PNT domain acts as a transcriptional activator.
- Information by UniProt
- E74 like factor 5 (ets domain transcription factor) antibody
- E74 like factor 5 antibody
- E74-like factor 5 antibody
ab13581 has been referenced in 2 publications.
- Chakrabarti R et al. Elf5 inhibits the epithelial-mesenchymal transition in mammary gland development and breast cancer metastasis by transcriptionally repressing Snail2. Nat Cell Biol 14:1212-22 (2012). IHC ; Human . PubMed: 23086238
- Krueger F et al. Down-regulation of Cdx2 in colorectal carcinoma cells by the Raf-MEK-ERK 1/2 pathway. Cell Signal 21:1846-56 (2009). PubMed: 19686845