Anti-ELFN2 antibody (ab224037)
Key features and details
- Rabbit polyclonal to ELFN2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ELFN2 antibody -
Description
Rabbit polyclonal to ELFN2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human ELFN2 aa 650 to the C-terminus.
Database link: Q5R3F8 -
Positive control
- IHC: Human cerebellum tissue. WB: U-2 OS cell lysate. ICC: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 90 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 90 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Relevance
ELFN2 contains 1 fibronectin type III domain and 5 LRR (leucine rich) repeats. The exact function of ELFN2 remains unknown. -
Cellular localization
Membrane; Single pass membrane protein. -
Database links
- Entrez Gene: 114794 Human
- Entrez Gene: 207393 Mouse
- SwissProt: Q5R3F8 Human
- SwissProt: Q68FM6 Mouse
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Alternative names
- dJ63G5.3 antibody
- ELFN 2 antibody
- ELFN2 antibody
see all
Images
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Paraffin-embedded human cerebellum tissue stained for ELFN2 using ab224037 at 1/50 dilution in immunohistochemical analysis
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Anti-ELFN2 antibody (ab224037) at 1/100 dilution + U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Predicted band size: 90 kDa -
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for ELFN2 (green) using ab224037 at 4 µg/ml in ICC/IF
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224037 has not yet been referenced specifically in any publications.