Key features and details
- Rabbit polyclonal to ELK1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ELK1 antibody
See all ELK1 primary antibodies
DescriptionRabbit polyclonal to ELK1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide conjugated to KLH, surrounding amino acids 387-391 (P-R-S-P-A) of Human ELK1 (NP_001107595.1).
- HeLa cells; 293 and HT29 cell extracts; Human breast carcinoma tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131465 was purified by affinity chromatography using epitope specific peptide.
Our Abpromise guarantee covers the use of ab131465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionStimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
Tissue specificityLung and testis.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
modificationsSumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
- Information by UniProt
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
All lanes : Anti-ELK1 antibody (ab131465) at 1/500 dilution
Lane 1 : 293 cell extracts
Lane 2 : HT29 cell extracts
Predicted band size: 45 kDa
Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed HeLa cells labelling ELK1 with ab131465 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ELK1 with ab131465 at 1/50 dilution.
ab131465 has been referenced in 3 publications.
- Chowdhury D et al. p38 MAPK pathway-dependent SUMOylation of Elk-1 and phosphorylation of PIAS2 correlate with the downregulation of Elk-1 activity in heat-stressed HeLa cells. Cell Stress Chaperones 24:393-407 (2019). PubMed: 30783905
- Gao Y et al. Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling. Commun Biol 2:359 (2019). PubMed: 31602408
- Legrand N et al. An AUG codon conserved for protein function rather than translational initiation: the story of the protein sElk1. PLoS One 9:e102890 (2014). WB ; Human . PubMed: 25036748