Overview

  • Product name

  • Description

    Rabbit polyclonal to ELK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding amino acids 387-391 (P-R-S-P-A) of Human ELK1 (NP_001107595.1).

  • Positive control

    • HeLa cells; 293 and HT29 cell extracts; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • Tissue specificity

    Lung and testis.
  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1, ETS transcription factor antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Images

  • All lanes : Anti-ELK1 antibody (ab131465) at 1/500 dilution

    Lane 1 : 293 cell extracts
    Lane 2 : HT29 cell extracts

    Predicted band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed HeLa cells labelling ELK1 with ab131465 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ELK1 with ab131465 at 1/50 dilution.

References

This product has been referenced in:

  • Chowdhury D  et al. p38 MAPK pathway-dependent SUMOylation of Elk-1 and phosphorylation of PIAS2 correlate with the downregulation of Elk-1 activity in heat-stressed HeLa cells. Cell Stress Chaperones 24:393-407 (2019). Read more (PubMed: 30783905) »
  • Legrand N  et al. An AUG codon conserved for protein function rather than translational initiation: the story of the protein sElk1. PLoS One 9:e102890 (2014). WB ; Human . Read more (PubMed: 25036748) »
See all 2 Publications for this product

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