Key features and details
- Rabbit polyclonal to ELK1
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ELK1 antibody
See all ELK1 primary antibodies
DescriptionRabbit polyclonal to ELK1
Tested applicationsSuitable for: IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ELK1 aa 200-250. The exact sequence is proprietary. NP_005220.2
Database link: P19419
- IP: HEK-293T whole cell lysate. IHC-P: Human breast carcinoma and prostate nodular hyperplasia tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245496 was affinity purified using an epitope specific to ELK1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionStimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
Tissue specificityLung and testis.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
modificationsSumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
- Information by UniProt
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
Formalin-fixed, paraffin-embedded human prostate nodular hyperplasia tissue stained for ELK1 using ab245496 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ELK1 using ab245496 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
ELK1 was immunoprecipitated from HEK-293T (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245496 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-ELK1 antibody at 1 µg/ml.
Lane 1: ab245496 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245496 has not yet been referenced specifically in any publications.