Overview

  • Product name

  • Description

    Rabbit polyclonal to ELK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ELK1 aa 200-250. The exact sequence is proprietary. NP_005220.2
    Database link: P19419

  • Positive control

    • IP: HEK-293T whole cell lysate. IHC-P: Human breast carcinoma and prostate nodular hyperplasia tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
    • Tissue specificity

      Lung and testis.
    • Sequence similarities

      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • Post-translational
      modifications

      Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
      On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ELK 1 antibody
      • Elk1 antibody
      • ELK1 member of ETS oncogene family antibody
      • ELK1 protein antibody
      • ELK1, ETS transcription factor antibody
      • ELK1_HUMAN antibody
      • ELK2 member of ETS oncogene family antibody
      • ETS domain containing protein Elk 1 antibody
      • ETS domain containing protein Elk1 antibody
      • ETS domain protein Elk1 antibody
      • ETS domain-containing protein Elk-1 antibody
      • ETS like gene 1 antibody
      • Member of ETS oncogene family antibody
      • Oncogene Elk1 antibody
      • Tyrosine kinase (ELK1) oncogene antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human prostate nodular hyperplasia tissue stained for ELK1 using ab245496 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ELK1 using ab245496 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    • ELK1 was immunoprecipitated from HEK-293T (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245496 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-ELK1 antibody at 1 µg/ml.

      Lane 1: ab245496 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    ab245496 has not yet been referenced specifically in any publications.

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