Key features and details
- Rabbit polyclonal to ELK1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ELK1 antibody
See all ELK1 primary antibodies
DescriptionRabbit polyclonal to ELK1
SpecificityAb27708 recognises Elk1.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length Elk1 protein (Human).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab27708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa.|
FunctionStimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
Tissue specificityLung and testis.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
modificationsSumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
- Information by UniProt
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
Anti-ELK1 antibody (ab27708) at 1 µg/ml + E. Coli
expressed Elk1 fusion protein.
Predicted band size: 45 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
The actual band size may differ from the predicted one as it is an E coli fusion protein.
ab27708 has not yet been referenced specifically in any publications.