Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-ELK1 antibody [E277] (ab32106)

Submit a review Q&A (1)References (25)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E277] to ELK1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ELK1 antibody [E277]
    See all ELK1 primary antibodies

  • Description

    Rabbit monoclonal [E277] to ELK1

  • Host species

    Rabbit

  • Specificity

    This antibody does not cross react with other ETS family members

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human ELK1 (C terminal).

  • Epitope

    ab32106 reacts with an epitope located in the C terminal region of ELK1.

  • Positive control

    • Human breast carcinoma ICC/IF: A549 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab32106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 62 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

Target

  • Function

    Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.

  • Tissue specificity

    Lung and testis.

  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.

  • Post-translational
    modifications

    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1, ETS transcription factor antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Images

  • Western blot - Anti-ELK1 antibody [E277] (ab32106)
    Western blot - Anti-ELK1 antibody [E277] (ab32106)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: ELK1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa positive whole cell lysate (21 µg)
    Lane 4: MCF7 negative whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32106 observed at 55 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab32106 was shown to specifically react with ELK1 in wild-type HAP1 cells as signal was lost in ELK1 knockout cells. Wild-type and ELK1 knockout samples were subjected to SDS-PAGE. Ab32106 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-ELK1 antibody [E277] (ab32106)
    Immunocytochemistry/ Immunofluorescence - Anti-ELK1 antibody [E277] (ab32106)

    Immunocytochemistry/ Immunofluorescence analysis of A549 (human lung carcinoma epithelial cell) cells labeling ELK1 with purified ab32106 at 1/500 dilution (3.8 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELK1 antibody [E277] (ab32106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELK1 antibody [E277] (ab32106)

    Immunohistochemical analysis of paraffin embedded human breast carcinoma using ab32106 at a dilution of 1/50

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-ELK1 antibody [E277] (ab32106)
    Flow Cytometry - Anti-ELK1 antibody [E277] (ab32106)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ELK1 with unpurified ab32106 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-ELK1 antibody [E277] (ab32106)
    Western blot - Anti-ELK1 antibody [E277] (ab32106)
    Anti-ELK1 antibody [E277] (ab32106) at 1/500 dilution
    Predicted band size: 45 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (25)

Publishing research using ab32106? Please let us know so that we can cite the reference in this datasheet.

ab32106 has been referenced in 25 publications.

  • Hou J  et al. Role of integrin-linked kinase in the hypoxia-induced phenotypic transition of pulmonary artery smooth muscle cells: Implications for hypoxic pulmonary hypertension. Exp Cell Res 382:111476 (2019). PubMed: 31255599
  • Gao Y  et al. Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling. Commun Biol 2:359 (2019). PubMed: 31602408
  • Sechet E  et al. Natural molecules induce and synergize to boost expression of the human antimicrobial peptide ß-defensin-3. Proc Natl Acad Sci U S A 115:E9869-E9878 (2018). PubMed: 30275324
  • Osmanbeyoglu HU  et al. Pancancer modelling predicts the context-specific impact of somatic mutations on transcriptional programs. Nat Commun 8:14249 (2017). PubMed: 28139702
  • Hipp N  et al. IL-2 imprints human naive B cell fate towards plasma cell through ERK/ELK1-mediated BACH2 repression. Nat Commun 8:1443 (2017). PubMed: 29129929
View all Publications for this product

Customer reviews and Q&As

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Question

The lot number of ELK-1 antibody is YJ060812DS.   

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Answer

The concentration of this lot is 0.2180 mg/ml

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