• Product name
    Anti-ELK1 antibody [E277] - ChIP Grade
    See all ELK1 primary antibodies
  • Description
    Rabbit monoclonal [E277] to ELK1 - ChIP Grade
  • Host species
  • Specificity
    This antibody does not cross react with other ETS family members
  • Tested applications
    Suitable for: WB, IHC-P, ICC, IP, Flow Cyt, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human ELK1 (C terminal).

  • Epitope
    ab32106 reacts with an epitope located in the C terminal region of ELK1.
  • Positive control
    • Human breast carcinoma
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 62 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50.
ICC 1/100.
IP 1/80.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP Use at an assay dependent concentration. PubMed: 22589737


  • Function
    Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • Tissue specificity
    Lung and testis.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1, ETS transcription factor antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all


  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: ELK1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa positive whole cell lysate (21 µg)
    Lane 4: MCF7 negative whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32106 observed at 55 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab32106 was shown to specifically react with ELK1 in wild-type HAP1 cells as signal was lost in ELK1 knockout cells. Wild-type and ELK1 knockout samples were subjected to SDS-PAGE. Ab32106 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma using ab32106 at a dilution of 1/50
  • Anti-ELK1 antibody [E277] - ChIP Grade (ab32106) at 1/500 dilution
    Predicted band size: 45 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ELK1 with unpurified ab32106 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.


This product has been referenced in:
  • Sechet E  et al. Natural molecules induce and synergize to boost expression of the human antimicrobial peptide ß-defensin-3. Proc Natl Acad Sci U S A 115:E9869-E9878 (2018). Read more (PubMed: 30275324) »
  • Osmanbeyoglu HU  et al. Pancancer modelling predicts the context-specific impact of somatic mutations on transcriptional programs. Nat Commun 8:14249 (2017). Read more (PubMed: 28139702) »
See all 23 Publications for this product

Customer reviews and Q&As


The concentration of this lot is 0.2180 mg/ml

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