Key features and details
- Rabbit polyclonal to ELK1 (phospho S383)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ELK1 (phospho S383) antibody
See all ELK1 primary antibodies
DescriptionRabbit polyclonal to ELK1 (phospho S383)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Mouse brain lysates and Human lung carcinoma tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab218133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/1000. Predicted molecular weight: 45 kDa.|
FunctionStimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
Tissue specificityLung and testis.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
modificationsSumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
- Information by UniProt
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
Anti-ELK1 (phospho S383) antibody (ab218133) at 1/300 dilution + mouse brain lysates
Developed using the ECL technique.
Predicted band size: 45 kDa
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human lung carcinoma tissue, labeling ELK1 (phospho S383) using ab218133 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab218133 has been referenced in 1 publication.
- Gao Y et al. Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling. Commun Biol 2:359 (2019). PubMed: 31602408