Anti-ELK1 (phospho S383) antibody (ab218133)
Key features and details
- Rabbit polyclonal to ELK1 (phospho S383)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ELK1 (phospho S383) antibody
See all ELK1 primary antibodies -
Description
Rabbit polyclonal to ELK1 (phospho S383) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human ELK1 aa 350 to the C-terminus (phospho S383) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P19419 -
Positive control
- Mouse brain lysates and Human lung carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/100 - 1/1000. Predicted molecular weight: 45 kDa.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/100 - 1/1000. Predicted molecular weight: 45 kDa. |
Target
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Function
Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. -
Tissue specificity
Lung and testis. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Post-translational
modificationsSumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2002 Human
- Entrez Gene: 13712 Mouse
- Entrez Gene: 314436 Rat
- Omim: 311040 Human
- SwissProt: P19419 Human
- SwissProt: P41969 Mouse
- SwissProt: A4GTP4 Rat
- Unigene: 181128 Human
see all -
Alternative names
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
see all
Images
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Anti-ELK1 (phospho S383) antibody (ab218133) at 1/300 dilution + mouse brain lysates
Developed using the ECL technique.
Predicted band size: 45 kDa -
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human lung carcinoma tissue, labeling ELK1 (phospho S383) using ab218133 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab218133 has been referenced in 2 publications.
- Gao Y et al. Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling. Commun Biol 2:359 (2019). PubMed: 31602408
- Shi W et al. EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells. J Biol Chem 294:6871-6887 (2019). PubMed: 30824540