Key features and details
- Rabbit polyclonal to ELK1 (phospho S383)
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Product nameAnti-ELK1 (phospho S383) antibody
See all ELK1 primary antibodies
DescriptionRabbit polyclonal to ELK1 (phospho S383)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Mouse, Rat, Dog, Human, Non human primates, Zebrafish
Synthetic peptide within Human ELK1 (phospho S383). The exact sequence is proprietary.
Database link: P19419
- WB: Recombinant ELK-1.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Constituents: HEPES, 0.87% Sodium chloride, 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionStimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
Tissue specificityLung and testis.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
modificationsSumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
- Information by UniProt
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254139 has not yet been referenced specifically in any publications.