Anti-ELK1 (phospho S383) antibody (ab34270)
Key features and details
- Rabbit polyclonal to ELK1 (phospho S383)
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-ELK1 (phospho S383) antibody
See all ELK1 primary antibodies -
Description
Rabbit polyclonal to ELK1 (phospho S383) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human ELK1 (phospho S383).
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Positive control
- Recombinant Elk1 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. -
Tissue specificity
Lung and testis. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Post-translational
modificationsSumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
see all
Images
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Anti-ELK1 (phospho S383) antibody (ab34270) at 1/1000 dilution + recombinant Elk1 protein
Predicted band size: 45 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab34270 has been referenced in 3 publications.
- Pérez-Rodríguez MJ et al. Mutant Huntingtin affects toll-like receptor 4 intracellular trafficking and cytokine production in mast cells. J Neuroinflammation 17:95 (2020). PubMed: 32220257
- Disser NP et al. Insulin-like growth factor 1 signaling in tenocytes is required for adult tendon growth. FASEB J 33:12680-12695 (2019). PubMed: 31536390
- Hou J et al. Role of integrin-linked kinase in the hypoxia-induced phenotypic transition of pulmonary artery smooth muscle cells: Implications for hypoxic pulmonary hypertension. Exp Cell Res 382:111476 (2019). PubMed: 31255599