Overview

  • Product name

  • Description

    Rabbit polyclonal to ELK3/NET
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide within Human ELK3/NET aa 252-301 (internal sequence). The exact sequence is proprietary.
    Sequence:

    LPSEHRSLFLEAACHDSDSLEPLNLSSGSKTKSPSLPPKAKKPKGLEISA

  • Positive control

    • Fetal heart lysate
  • General notes

    Protein previously labeled as ELK3.

Properties

Applications

Our Abpromise guarantee covers the use of ab83186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-ELK3/NET antibody (ab83186) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa
    Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.

References

ab83186 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Gel Running Conditions
Non-reduced Denaturing (4-12% gradient Bis-Tris gel)
Loading amount
15 µg
Specification
NIH 3T3 cells
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 22 2010

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