Product nameAnti-ELK4 antibody
DescriptionRabbit polyclonal to ELK4
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
Three synthetic peptides derived from human ELK4, conjugated to KLH.
- An anti thio tested on an induced culture of E. coli.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Concentration information loading...
Purification notesCrude serum, final bleed
Our Abpromise guarantee covers the use of ab86002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 47 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionForms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
- Information by UniProt
- Elk4 antibody
- ELK4_HUMAN antibody
- ETS domain containing protein Elk4 antibody
All lanes : Anti-ELK4 antibody (ab86002) at 1/1000 dilution (in 5% non-fat milk-PBST solution incubated for 60 minutes)
Lane 1 : uninduced culture of E. coli (plus anti thio at 1/5000 dilution)
Lane 2 : induced culture of E. coli (plus anti thio at 1/5000 dilution)
All lanes : anti-Rabbit IgG HRP conjugated incubated for 60 minutes at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted?
Gel concentration: 10%
The actual band molecular weight is the result of the target protein ELK4 (47 kDa) attached to a tag (14 kDa).
ab86002 has not yet been referenced specifically in any publications.