Overview

  • Product name

    Anti-ELKS antibody [EPR13777]
    See all ELKS primary antibodies
  • Description

    Rabbit monoclonal [EPR13777] to ELKS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human ELKS aa 700-850 (internal sequence).
    Database link: Q8IUD2

  • Positive control

    • HeLa, HEK293, Jurkat and SW480 cell lysate. SW480 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).
ICC/IF 1/250.

Target

  • Function

    Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport.
  • Tissue specificity

    Widely expressed. Isoform 2 and isoform 4 are abundantly expressed in brain. Isoform 1 and isoform 3 are predominantly expressed in testis and thyroid, and isoform 1 predominates in other tissues tested.
  • Involvement in disease

    Thyroid papillary carcinoma (TPC) [MIM:188550]: A common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=The gene represented in this entry may be involved in disease pathogenesis. A chromosomal aberration involving ERC1/RAB6IP2 is found in cause of thyroid papillary carcinomas. Translocation t(10;12)(q11;p13) involving RET. In vitro, isoform 1, isoform 3 and isoform 5 participating in a ERC1-RET fusion protein activate tyrosine-protein kinase activity.
  • Sequence similarities

    Contains 1 FIP-RBD domain.
  • Cellular localization

    Cytoplasm. Membrane. Golgi apparatus membrane. In neurons, localized closed to presynaptic membrane. Recruited on Golgi membranes by RAB6A in a GTP-dependent manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cast 2 antibody
    • CAST2 antibody
    • CAZ associated structural protein 2 antibody
    • Elks antibody
    • ELKS/RAB6 interacting/CAST family member 1 antibody
    • ELKS/RAB6-interacting/CAST family member 1 antibody
    • ERC 1 antibody
    • ERC protein 1 antibody
    • ERC-1 antibody
    • Erc1 antibody
    • ERC1 protein antibody
    • KIAA1081 antibody
    • MGC12974 antibody
    • RAB6 interacting protein 2 antibody
    • RAB6-interacting protein 2 antibody
    • RAB6IP2 antibody
    • RB6I2_HUMAN antibody
    see all

Images

  • All lanes : Anti-ELKS antibody [EPR13777] (ab180507) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK293 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : SW480 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 128 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

  • Immunofluorescence analysis of HeLa cells labeling ELKS (red) with ab180507 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/250 dilution. Nuclei were counterstained with DAPI (blue).

References

This product has been referenced in:

  • Tirosh O  et al. The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions. PLoS Pathog 11:e1005288 (2015). WB . Read more (PubMed: 26599541) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

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Verified customer

Submitted May 17 2019

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