Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13777] to ELKS
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-ELKS antibody [EPR13777]
See all ELKS primary antibodies
DescriptionRabbit monoclonal [EPR13777] to ELKS
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human ELKS aa 700-850 (internal sequence).
Database link: Q8IUD2
- HeLa, HEK293, Jurkat and SW480 cell lysate. SW480 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).|
FunctionRegulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport.
Tissue specificityWidely expressed. Isoform 2 and isoform 4 are abundantly expressed in brain. Isoform 1 and isoform 3 are predominantly expressed in testis and thyroid, and isoform 1 predominates in other tissues tested.
Involvement in diseaseThyroid papillary carcinoma (TPC) [MIM:188550]: A common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=The gene represented in this entry may be involved in disease pathogenesis. A chromosomal aberration involving ERC1/RAB6IP2 is found in cause of thyroid papillary carcinomas. Translocation t(10;12)(q11;p13) involving RET. In vitro, isoform 1, isoform 3 and isoform 5 participating in a ERC1-RET fusion protein activate tyrosine-protein kinase activity.
Sequence similaritiesContains 1 FIP-RBD domain.
Cellular localizationCytoplasm. Membrane. Golgi apparatus membrane. In neurons, localized closed to presynaptic membrane. Recruited on Golgi membranes by RAB6A in a GTP-dependent manner.
- Information by UniProt
- Cast 2 antibody
- CAST2 antibody
- CAZ associated structural protein 2 antibody
All lanes : Anti-ELKS antibody [EPR13777] (ab180507) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 128 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Blocking buffer: 5% NFDM/TBST
Immunofluorescence analysis of HeLa cells labeling ELKS (red) with ab180507 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/250 dilution. Nuclei were counterstained with DAPI (blue).
ab180507 has been referenced in 1 publication.
- Tirosh O et al. The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions. PLoS Pathog 11:e1005288 (2015). WB . PubMed: 26599541