Overview

  • Product name

  • Description

    Rabbit polyclonal to ELL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human ELL aa 150-200. The exact sequence is proprietary. NP_006523.1
    Database link: P55199

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance

      ELL was shown to encode an elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. ELL is the second elongation factor to be implicated in oncogenesis.
    • Cellular localization

      Nuclear. Colocalizes with EAF2 to nuclear speckles and to Cajal bodies.
    • Database links

    • Alternative names

      • eleven nineteen lysine rich leukemia gene antibody
      • Eleven-nineteen lysine-rich leukemia protein antibody
      • ELL1 antibody
      • elongation factor RNA polymerase II antibody
      • Men antibody
      • RNA polymerase II elongation factor antibody
      see all

    Images

    • ELL was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

      ab245592 used for IP at 3 µg/mg lysate. For WB another anti-ELL antibody was used at 1 µg/ml.

      Lane 1: ab245592 IP in HeLa whole cell lysate.
      Lane 2: Control IgG in HeLa whole cell lysate.

      Chemiluminescence detection: 30 seconds.

    References

    ab245592 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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