Key features and details
- Rabbit polyclonal to ELL2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ELL2 antibody
See all ELL2 primary antibodies
DescriptionRabbit polyclonal to ELL2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
Synthetic peptide, corresponding to a region within amino acids 400-450 of Human ELL2 (NP_036213.2).
- HeLa whole cell lysate (ab150035)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab115027 was affinity purified using an epitope specific to ELL2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab115027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 72 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionElongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.
Sequence similaritiesBelongs to the ELL/occludin family.
- Information by UniProt
- ELL related RNA polymerase II elongation factor antibody
- Ell2 antibody
- ELL2_HUMAN antibody
All lanes : Anti-ELL2 antibody (ab115027) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 30 seconds
Detection of ELL2 by Western Blot of Immunprecipitate.
ab115027, at 1 µg/ml, staining ELL2 in Hela whole cell lysate immunoprecipitated using ab115027 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab115027 has been referenced in 1 publication.
- Kuzmina A et al. Super elongation complex promotes early HIV transcription and its function is modulated by P-TEFb. Transcription 8:133-149 (2017). PubMed: 28340332