Key features and details
- Mouse polyclonal to ELL3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ELL3 antibody
See all ELL3 primary antibodies
DescriptionMouse polyclonal to ELL3
SpecificityReactive against mammalian transfected lysate.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein, corresponding to amino acids 1-398 of Human ELL3
- Transfected 293T lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa).|
RelevanceELL3 is a testis-specific RNA polymerase II elongation factor which increases the catalytic rate of transcription elongation. ELL3 is approximately 50% similar to both ELL and ELL2.
- Elongation Factor RNA Polymerase II-Like 3 antibody
- RNA polymerase II elongation factor ELL3 antibody
All lanes : Anti-ELL3 antibody (ab67415) at 1/500 dilution
Lane 1 : ELL3 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 45 kDa
Observed band size: 43.78 kDa why is the actual band size different from the predicted?
ab67415 has been referenced in 2 publications.
- Lee JY et al. Ell3 functions as a critical decision maker at the crossroad between stem cell senescence and apoptosis. Stem Cell Res Ther 10:32 (2019). PubMed: 30654843
- Ahn HJ et al. Ell3 stabilizes p53 following CDDP treatment via its effects on ubiquitin-dependent and -independent proteasomal degradation pathways in breast cancer cells. Oncotarget 6:44523-37 (2015). WB . PubMed: 26540344