• Product name
  • Description
    Goat polyclonal to ELMO1
  • Host species
  • Specificity
    This antibody is expected to recognise both human isoforms.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Zebrafish
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 714-727 of Human ELMO1.

  • Positive control
    • Jurkat cell lysate.



Our Abpromise guarantee covers the use of ab2239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 18662984
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Can be blocked with Human ELMO1 peptide (ab22878).

Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa).

IP Use at an assay dependent concentration.


  • Function
    Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
  • Tissue specificity
    Widely expressed, with a higher expression in the spleen and placenta.
  • Sequence similarities
    Contains 1 ELMO domain.
    Contains 1 PH domain.
  • Post-translational
    Phosphorylated by HCK.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
  • Information by UniProt
  • Database links
  • Alternative names
    • CED 12 antibody
    • Ced 12 homolog 1 antibody
    • Ced 12 homolog antibody
    • CED-12 antibody
    • CED12 antibody
    • Ced12 homolog 1 antibody
    • Ced12 homolog antibody
    • ELMO 1 antibody
    • ELMO-1 antibody
    • Elmo1 antibody
    • ELMO1_HUMAN antibody
    • Engulfment and cell motility 1 antibody
    • Engulfment and cell motility protein 1 antibody
    • KIAA0281 antibody
    • MGC126406 antibody
    • Protein ced-12 homolog antibody
    see all


  • All lanes : Anti-ELMO1 antibody (ab2239) at 0.3 µg/ml

    Lane 1 : Human Frontal Cortex cell lysate (35µg protein in RIPA buffer).
    Lane 2 : Mouse cell lysate (35µg protein in RIPA buffer).
    Lane 3 : Rat cell lysate (35µg protein in RIPA buffer).

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • IHC image of ab2239 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2239, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Ab2239 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Ab2239 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  •     Lane 1-3: whole cell lysate (differentiated HL60 cells)

    Lane 4: Protein molculare standard (100, 75 and 50kDa)

    Lane 5: Immunoprecipitation with normal IgG (mock)

    Lane 6 and 7: Immunoprecipitation with ab2239 antibody

    Immunoprecipiated with ab2239 1.5 ug/sample (about 400 ug total protein). Immunoblotted with polyclonal rabbit anti-Dock2  or ab2239 antibody.

    See Abreview

  • ab2239 staining ELMO1 in human HL60 cells by Immunocytochemistry/ Immunofluoresecence.  Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized in 0.2% Triton X-100 in PBS for 5 minutes, blocked in 10% normal donkey serum for 30 minutes and incubated with primary antibodies for 2 hours at room temperature. After washing three times with 0.1% Tween 20 in PBS, the coverslips were incubated with fluorescence-conjugated secondary antibodies for 1 hour.


This product has been referenced in:
  • Makino Y  et al. Tyr724 phosphorylation of ELMO1 by Src is involved in cell spreading and migration via Rac1 activation. Cell Commun Signal 13:35 (2015). WB . Read more (PubMed: 26205662) »
  • Capala ME  et al. ELMO1 is upregulated in AML CD34+ stem/progenitor cells, mediates chemotaxis and predicts poor prognosis in normal karyotype AML. PLoS One 9:e111568 (2014). WB ; Human . Read more (PubMed: 25360637) »
See all 10 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for your inquiry.
I do believe the band you're seeing at 50 kDa is specific for ELMO1. The protein has 3 isoforms, according to SwissProt, at 84, 50, and 29 kDa.
So it may be that your samples are only expressing the 50 kDa isoform. You could run a positive control with Jurkat lysate to see if this is the case. It may be that Jurkats express both larger isoforms while bladder epithelial cells only express the 50 kDa isoform, but this would have to be confirmed with further study and consultation of the literature.
I hope this information helps. Please contact us with any other questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell lysate - whole cell (differentiated HL60)
Total protein in input
1.5e+007 cells
differentiated HL60
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

Submitted Apr 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (differentiated HL60)
Loading amount
20 µg
differentiated HL60
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Mr. Jiqing Sai

Verified customer

Submitted Feb 21 2007


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