Product nameAnti-ELMO1 antibody
See all ELMO1 primary antibodies
DescriptionRabbit polyclonal to ELMO1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Synthetic peptide corresponding to Human ELMO1 aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Jurkat and MOLT4 Whole Cell Lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).|
FunctionInvolved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Tissue specificityWidely expressed, with a higher expression in the spleen and placenta.
Sequence similaritiesContains 1 ELMO domain.
Contains 1 PH domain.
modificationsPhosphorylated by HCK.
Cellular localizationCytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
- Information by UniProt
- CED 12 antibody
- Ced 12 homolog 1 antibody
- Ced 12 homolog antibody
All lanes : Anti-ELMO1 antibody (ab72859) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 50 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab72859 has not yet been referenced specifically in any publications.