Overview

  • Product name

    Anti-ELMO1 antibody - C-terminal
    See all ELMO1 primary antibodies
  • Description

    Rabbit polyclonal to ELMO1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 664-727 of Human ELMO1 (Uniprot ID Q92556).

  • Positive control

    • H1299 whole cell lysate; HeLa cells; HepG2 and A431 cell line; Human Stomach tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 84 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
  • Tissue specificity

    Widely expressed, with a higher expression in the spleen and placenta.
  • Sequence similarities

    Contains 1 ELMO domain.
    Contains 1 PH domain.
  • Post-translational
    modifications

    Phosphorylated by HCK.
  • Cellular localization

    Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
  • Information by UniProt
  • Database links

  • Alternative names

    • CED 12 antibody
    • Ced 12 homolog 1 antibody
    • Ced 12 homolog antibody
    • CED-12 antibody
    • CED12 antibody
    • Ced12 homolog 1 antibody
    • Ced12 homolog antibody
    • ELMO 1 antibody
    • ELMO-1 antibody
    • Elmo1 antibody
    • ELMO1_HUMAN antibody
    • Engulfment and cell motility 1 antibody
    • Engulfment and cell motility protein 1 antibody
    • KIAA0281 antibody
    • MGC126406 antibody
    • Protein ced-12 homolog antibody
    see all

Images

  • Anti-ELMO1 antibody - C-terminal (ab155775) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 84 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa, labeling ELMO1 with ab155775 at 1/500 dilution. Blue: Hoechst 33342 staining.
  • Immunohistochemical analysis of paraffin-embedded Human Stomach tissue, labeling ELMO1 with ab155775 at 1/100 dilution.

References

ab155775 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155775.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up