Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12919] to ELMO1
- Suitable for: ICC/IF, WB, IP, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-ELMO1 antibody [EPR12919]
See all ELMO1 primary antibodies
DescriptionRabbit monoclonal [EPR12919] to ELMO1
Tested applicationsSuitable for: ICC/IF, WB, IP, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ELMO1 aa 700 to the C-terminus. The exact sequence is proprietary.
Database link: Q92556
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 84 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||Use at an assay dependent concentration.|
FunctionInvolved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Tissue specificityWidely expressed, with a higher expression in the spleen and placenta.
Sequence similaritiesContains 1 ELMO domain.
Contains 1 PH domain.
modificationsPhosphorylated by HCK.
Cellular localizationCytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
- Information by UniProt
- CED 12 antibody
- Ced 12 homolog 1 antibody
- Ced 12 homolog antibody
Immunocytochemistry/ Immunofluorescence analysis of K562 labeling ELMO1 with ab174298 at 1/250 dilution. Goat anti rabbit IgG(Alexa Fluor® 488); ab150077 at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to counter stain nuclei (blue).
All lanes : Anti-ELMO1 antibody [EPR12919] (ab174298) at 1/10000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 84 kDa
LR73 cells were transfected with the indicated plasmids and stained with Alexa Fluor 488–conjugated phalloidin and anti-Elmo1 antibody.
One day after transfection, cells were washed with cold PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100 for 5 min. Next, permeabilized cells were blocked with 1% BSA for 30 min and stained with Alex Fluor 594–conjugated phalloidin and anti-Elmo1 antibody for 1 h at room temperature.
(After Figure 5 of Kim et al).
Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling ELMO1 with unpurified ab174298 at 1:200 dilution(1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab174298 at 1/10 dilution.
ab174298 has been referenced in 2 publications.
- Liang Y et al. Downregulation of Dock1 and Elmo1 suppresses the migration and invasion of triple-negative breast cancer epithelial cells through the RhoA/Rac1 pathway. Oncol Lett 16:3481-3488 (2018). PubMed: 30127952
- Kim K et al. Intermolecular steric inhibition of Ephexin4 is relieved by Elmo1. Sci Rep 7:4404 (2017). PubMed: 28667327