Overview

  • Product name

    Anti-ELMO1 antibody [EPR12919]
    See all ELMO1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12919] to ELMO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ELMO1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q92556

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899); human placenta lysate. IP: Jurkat whole cell lysate (ab7899). Flow cyt: Jurkat cells. ICC/IF: K562 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Predicted molecular weight: 84 kDa.
IP 1/10 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
    • Tissue specificity

      Widely expressed, with a higher expression in the spleen and placenta.
    • Sequence similarities

      Contains 1 ELMO domain.
      Contains 1 PH domain.
    • Post-translational
      modifications

      Phosphorylated by HCK.
    • Cellular localization

      Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
    • Information by UniProt
    • Database links

    • Alternative names

      • CED 12 antibody
      • Ced 12 homolog 1 antibody
      • Ced 12 homolog antibody
      • CED-12 antibody
      • CED12 antibody
      • Ced12 homolog 1 antibody
      • Ced12 homolog antibody
      • ELMO 1 antibody
      • ELMO-1 antibody
      • Elmo1 antibody
      • ELMO1_HUMAN antibody
      • Engulfment and cell motility 1 antibody
      • Engulfment and cell motility protein 1 antibody
      • KIAA0281 antibody
      • MGC126406 antibody
      • Protein ced-12 homolog antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of K562 labeling ELMO1 with ab174298 at 1/250 dilution. Goat anti rabbit IgG(Alexa Fluor® 488); ab150077 at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to counter stain nuclei (blue).

    • All lanes : Anti-ELMO1 antibody [EPR12919] (ab174298) at 1/10000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 84 kDa

    • LR73 cells were transfected with the indicated plasmids and stained with Alexa Fluor 488–conjugated phalloidin and anti-Elmo1 antibody.

      One day after transfection, cells were washed with cold PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100 for 5 min. Next, permeabilized cells were blocked with 1% BSA for 30 min and stained with Alex Fluor 594–conjugated phalloidin and anti-Elmo1 antibody for 1 h at room temperature.

      (After Figure 5 of Kim et al).

      http://creativecommons.org/licenses/by/4.0/.

    • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling ELMO1 with unpurified ab174298 at 1:200 dilution(1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab174298 at 1/10 dilution.

    References

    This product has been referenced in:

    • Liang Y  et al. Downregulation of Dock1 and Elmo1 suppresses the migration and invasion of triple-negative breast cancer epithelial cells through the RhoA/Rac1 pathway. Oncol Lett 16:3481-3488 (2018). Read more (PubMed: 30127952) »
    • Kim K  et al. Intermolecular steric inhibition of Ephexin4 is relieved by Elmo1. Sci Rep 7:4404 (2017). Read more (PubMed: 28667327) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Filter by Application

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    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Stomach)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: PH6
    Permeabilization
    Yes - 0.1% triton
    Specification
    Stomach
    Blocking step
    1% BSA, Goat serum, donkey serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 18 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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