Overview

  • Product name

    Anti-ELMO1 antibody [EPR12919] - BSA and Azide free
    See all ELMO1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12919] to ELMO1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ELMO1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q92556

  • Positive control

    • Human placenta and Jurkat lysates. ICC/IF: K562 cells
  • General notes

    Ab222227 is the carrier-free version of ab174298. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab222227 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
    • Tissue specificity

      Widely expressed, with a higher expression in the spleen and placenta.
    • Sequence similarities

      Contains 1 ELMO domain.
      Contains 1 PH domain.
    • Post-translational
      modifications

      Phosphorylated by HCK.
    • Cellular localization

      Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
    • Information by UniProt
    • Database links

    • Alternative names

      • CED 12 antibody
      • Ced 12 homolog 1 antibody
      • Ced 12 homolog antibody
      • CED-12 antibody
      • CED12 antibody
      • Ced12 homolog 1 antibody
      • Ced12 homolog antibody
      • ELMO 1 antibody
      • ELMO-1 antibody
      • Elmo1 antibody
      • ELMO1_HUMAN antibody
      • Engulfment and cell motility 1 antibody
      • Engulfment and cell motility protein 1 antibody
      • KIAA0281 antibody
      • MGC126406 antibody
      • Protein ced-12 homolog antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of K562 labeling ELMO1 with ab222227 at 1/250 dilution. Goat anti rabbit IgG(Alexa Fluor® 488); ab150077 at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to counter stain nuclei (blue).

    • LR73 cells were transfected with the indicated plasmids and stained with Alexa Fluor 488–conjugated phalloidin and anti-Elmo1 antibody.

      One day after transfection, cells were washed with cold PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100 for 5 min. Next, permeabilized cells were blocked with 1% BSA for 30 min and stained with Alex Fluor 594–conjugated phalloidin and anti-Elmo1 antibody for 1 h at room temperature.

      (After Figure 5 of Kim et al).

      http://creativecommons.org/licenses/by/4.0/.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174298).

    • This Flow Cyt data was generated using the same atni-ELMO1 antibody clone, EPR12919, in a different buffer formulation (cat# ab174298).

      Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling ELMO1 with unpurified ab174298 at 1:200 dilution(1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • This IP data was generated using the same atni-ELMO1 antibody clone, EPR12919, in a different buffer formulation (cat# ab174298).

      Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab174298 at 1/10 dilution.

    References

    ab222227 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab222227.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up