Product nameELMO2 overexpression 293T lysate (whole cell)
ab94207 is a 293T cell transfected lysate in which Human ELMO2 has been transiently over-expressed using a pCMV-ELMO2 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
BackgroundFunction: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. Tissue specificity: Widely expressed, with a higher expression in skeletal muscle, kidney and placenta. Similarity: Contains 1 ELMO domain. Contains 1 PH domain.
Our Abpromise guarantee covers the use of ab94207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94207 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-ELMO2 antibody (ab57557) at 1/500 dilution
Lane 1 :
ELMO2 overexpression 293T lysate (whole cell) (ab94207)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94207 has not yet been referenced specifically in any publications.