Anti-ELOF1 antibody (ab130402)
Key features and details
- Rabbit polyclonal to ELOF1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ELOF1 antibody -
Description
Rabbit polyclonal to ELOF1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment: KMTGTLETQF TCPFCNHEKS CDVKMDRARN TGVISCTVCL EEFQTPITYL SEPVDVYSDW, corresponding to amino acids 15-74 of Human ELOF1 (P60002).
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Positive control
- Human lateral ventricle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions. -
Sequence similarities
Belongs to the ELOF1 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 84337 Human
- SwissProt: P60002 Human
- Unigene: 631633 Human
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Alternative names
- ELF1 antibody
- elof1 antibody
- ELOF1_HUMAN antibody
see all
Images
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab130402 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human lateral ventricle tissue labelled with ELOF1 antibody at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab130402 has not yet been referenced specifically in any publications.