Overview

  • Product name

    Anti-ELOF1 antibody
  • Description

    Rabbit polyclonal to ELOF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment: KMTGTLETQF TCPFCNHEKS CDVKMDRARN TGVISCTVCL EEFQTPITYL SEPVDVYSDW, corresponding to amino acids 15-74 of Human ELOF1 (P60002).

  • Positive control

    • Human lateral ventricle tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab130402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.
  • Sequence similarities

    Belongs to the ELOF1 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ELF1 antibody
    • elof1 antibody
    • ELOF1_HUMAN antibody
    • Elongation factor 1 homolog (ELF1, S. cerevisiae) antibody
    • Elongation factor 1 homolog (S. cerevisiae) antibody
    • Transcription elongation factor 1 homolog antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab130402 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human lateral ventricle tissue labelled with ELOF1 antibody at 1/10 dilution.

References

ab130402 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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