• Product name

    Anti-Elongation factor 1-gamma antibody
    See all Elongation factor 1-gamma primary antibodies
  • Description

    Rabbit polyclonal to Elongation factor 1-gamma
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human, Monkey
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human Elongation factor 1-gamma aa 200-250.
    Database link: NP_001395.1

  • Positive control

    • Whole cell lysate from Hela, 293T and NIH3T3 cells.
  • General notes

    Previously labelled as EEF1G. 


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab72368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function

    Probably plays a role in anchoring the complex to other cellular components.
  • Tissue specificity

    Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.
  • Sequence similarities

    Contains 1 EF-1-gamma C-terminal domain.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610301D06Rik antibody
    • AA407312 antibody
    • eEF 1B gamma antibody
    • EEF 1G antibody
    • eEF-1B gamma antibody
    • EEF1G antibody
    • EF 1 gamma antibody
    • EF 1G antibody
    • EF-1-gamma antibody
    • EF1 gamma antibody
    • EF1G antibody
    • EF1G_HUMAN antibody
    • Elongation factor 1 gamma antibody
    • Elongation factor 1-gamma antibody
    • Eukaryotic translation elongation factor 1 gamma antibody
    • GIG 35 antibody
    • GIG35 antibody
    • MGC103354 antibody
    • MGC114210 antibody
    • MGC144723 antibody
    • MGC144724 antibody
    • MGC94929 antibody
    • Pancreatic tumor related protein antibody
    • PRO1608 antibody
    • Translation elongation factor eEF 1 gamma chain antibody
    see all


  • All lanes : Anti-Elongation factor 1-gamma antibody (ab72368) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Elongation factor 1-gamma with ab72368 at 1/200 (1µg/ml). Detection: DAB.

  • Immunoprecipitation/ Western Blot of Elongation factor 1-gamma. Lane 1: ab72368 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72368 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 30 seconds.

  • All lanes : Anti-Elongation factor 1-gamma antibody (ab72368) at 1/3000 dilution (in PBS-T for 16 hours at 4°C)

    Lane 1 : Monkey kidney whole cell lysate overexpressing Elongation factor 1-gamma
    Lane 2 : COS7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 10 seconds

    Blocking Step: 5% Milk for 1 hour at 22°C

    See Abreview

  • IHC image of ab72368 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab72368 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72368, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Pellegrini L  et al. Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice. Hum Mol Genet 27:3257-3271 (2018). Read more (PubMed: 29917075) »
  • Ayre DC  et al. CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo. Sci Rep 7:8642 (2017). Read more (PubMed: 28819186) »
See all 5 Publications for this product

Customer reviews and Q&As

Western blot
Monkey Cell lysate - whole cell (kidney)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

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Submitted Jul 14 2010

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