Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7200] to Elongation factor 1-gamma
- Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-Elongation factor 1-gamma antibody [EPR7200]
See all Elongation factor 1-gamma primary antibodies
DescriptionRabbit monoclonal [EPR7200] to Elongation factor 1-gamma
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Elongation factor 1-gamma aa 50-150. The exact sequence is proprietary.
- 293T, HL60, C6, RAW 264.7, PC12, NIH 3T3, HepG2 and HeLa cell lysates; Human pancreatic adenocarcinoma tissue; HeLa cells.
Previously labelled as EEF1G.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.31 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
|Flow Cyt||1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionProbably plays a role in anchoring the complex to other cellular components.
Tissue specificityHighly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.
Sequence similaritiesContains 1 EF-1-gamma C-terminal domain.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain.
- Information by UniProt
- 2610301D06Rik antibody
- AA407312 antibody
- eEF 1B gamma antibody
All lanes : Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH 3T3 cell lysate
Lane 7 : HepG2 cell lysate
Lane 8 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa
Overlay histogram showing HeLa cells stained with ab124994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124994, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab124994 at 1/250 dilution staining Elongation factor 1-gamma in paraffin-embedded Human pancreatic adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab124994 at 1/100 dilution staining Elongation factor 1-gamma in HeLa cells by Immunofluorescence.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124994 has been referenced in 2 publications.
- Sammaibashi S et al. Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins. Front Microbiol 9:1446 (2018). PubMed: 30008712
- Milone MR et al. Proteomic analysis of zoledronic-acid resistant prostate cancer cells unveils novel pathways characterizing an invasive phenotype. Oncotarget 6:5324-41 (2015). WB . PubMed: 25481874