Overview

  • Product name

  • Description

    Rabbit polyclonal to Elongin A
  • Host species

    Rabbit
  • Specificity

    ab227384 detects endogenous levels of total Elongin A protein.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Elongin A aa 131-180. The exact sequence is proprietary.
    Database link: Q14241

  • Positive control

    • IHC-P: Human skin and liver tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 90 kDa.
IHC-P 1/50.

Target

  • Function

    SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 1 TFIIS N-terminal domain.
  • Domain

    The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ela1 antibody
    • Elo A antibody
    • EloA antibody
    • ELOA1_HUMAN antibody
    • Elongin 110 kDa subunit antibody
    • Elongin-A antibody
    • ElonginA antibody
    • RNA polymerase II transcription factor SIII subunit A1 antibody
    • SIII antibody
    • SIII p110 antibody
    • TCEB 3 antibody
    • Tceb3 antibody
    • TCEB3A antibody
    • Transcription elongation factor B (SIII) polypeptide 3 (110kD) antibody
    • Transcription elongation factor B (SIII) polypeptide 3 antibody
    • Transcription elongation factor B alpha subunit antibody
    • Transcription elongation factor B polypeptide 3 antibody
    see all

Images

  • All lanes : Anti-Elongin A antibody (ab227384) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
    Lane 2 : HUVEC (human umbilical vein endothelial cell line) cell extract
    Lane 3 : HUVEC cell extract with immunizing peptide

    Predicted band size: 90 kDa

  • Formalin-fixed, paraffin-embedded human skin tissue stained for Elongin A using ab227384 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Elongin A using ab227384 at 1/50 dilution in immunohistochemical analysis.

References

ab227384 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227384.
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