Key features and details
- Rabbit polyclonal to Elongin A
- Suitable for: WB, IHC-P
- Isotype: IgG
Product nameAnti-Elongin A antibody
See all Elongin A primary antibodies
DescriptionRabbit polyclonal to Elongin A
Tested applicationsSuitable for: WB, IHC-Pmore details
Recombinant fragment (His-T7-tag) corresponding to Mouse Elongin A aa 76-345. (Expressed in E.coli)
VPVERNSEAEDQDFEKNNSRKRPRDALQREEELEGNYQESWKPSGSRSYS PEHRQKKHKKLSEPERPHKVAHSHEKRDERKRCHKVSPPYSSDPESSDYG HVQSPPPSSPHQMYTDLSRSPEEDQEPIISHQKPGKVHSNTFQDRLGVSH LGEQGKGAVSHHKQHRSSHKEKHPADAREDEKISAVSREKSHKASSKEES RRLLSGDSAKEKLPSSVVKKDKDREGSSLKKKFSPALDVASDNHFKKPKH KDSEKAKSDKNKQSVDGVDS
Database link: Q8CB77
- IHC-P: Mouse liver, spleen and vas deferens tissues. WB: Mouse liver and brain lysates; HeLa cell lysate; Recombinant mouse Elongin A protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231586 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 87 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionSIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
Sequence similaritiesContains 1 F-box domain.
Contains 1 TFIIS N-terminal domain.
DomainThe elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
- Information by UniProt
- Ela1 antibody
- Elo A antibody
- EloA antibody
All lanes : Anti-Elongin A antibody (ab231586) at 3 µg/ml
Lane 1 : Mouse liver lysate
Lane 2 : Mouse brain lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 87 kDa
Anti-Elongin A antibody (ab231586) at 3 µg/ml + Recombinant mouse Elongin A protein
HRP-Linked Guinea pig Anti-Rabbi at 1/2000 dilution
Predicted band size: 87 kDa
Paraffin-embedded mouse vas deferens tissue stained for Elongin A using ab231586 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded mouse spleen tissue stained for Elongin A using ab231586 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded mouse liver tissue stained for Elongin A using ab231586 at 20 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231586 has not yet been referenced specifically in any publications.