Overview

  • Product name

  • Description

    Rabbit polyclonal to Elongin-C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Cat, Xenopus laevis, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human Elongin-C (internal sequence). The exact sequence is proprietary.
    Database link: Q15369

  • Positive control

    • WB: PC-3, Jurkat, Raji and NCI-H929 cell lysate.
  • General notes

    Previously labelled as TCEB1. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 12 kDa.

Target

  • Function

    SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
    The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.
  • Tissue specificity

    Overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3.
  • Sequence similarities

    Belongs to the SKP1 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Elo C antibody
    • EloC antibody
    • ELOC_HUMAN antibody
    • Elongin 15 kDa subunit antibody
    • Elongin C antibody
    • Elongin-C antibody
    • ElonginC antibody
    • RNA polymerase II transcription factor SIII subunit C antibody
    • SIII antibody
    • SIII p15 antibody
    • TCEB 1 antibody
    • tceb1 antibody
    • Transcription elongation factor B (SIII) polypeptide 1 antibody
    • Transcription elongation factor B polypeptide 1 antibody
    see all

Images

  • Anti-Elongin-C antibody (ab226831) at 1/1000 dilution + PC-3 (human prostate adenocarcinoma cell line) cell lysate at 30 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 12 kDa

  • All lanes : Anti-Elongin-C antibody (ab226831) at 1/2000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) cell lysate
    Lane 3 : NCI-H929 cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 12 kDa

References

ab226831 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226831.
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