Recombinant Anti-ELOVL2 antibody [EPR11880] (ab176327)
![Immunoprecipitation - Anti-ELOVL2 antibody [EPR11880] (ab176327)](https://www.abcam.com/ps/products/176/ab176327/Images/ab176327-204125-anti-elovl2-antibody-epr11880-immunoprecipitation.jpg "Immunoprecipitation - Anti-ELOVL2 antibody [EPR11880] (ab176327)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11880] to ELOVL2
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ELOVL2 antibody [EPR11880]
See all ELOVL2 primary antibodies -
Description
Rabbit monoclonal [EPR11880] to ELOVL2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ELOVL2 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NXB9 -
Positive control
- Rat liver, Mouse testis, Fetal liver and Mouse liver lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11880 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab176327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/10 - 1/100. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 34 kDa. |
Target
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Function
Condensing enzyme that catalyzes the synthesis of polyunsaturated very long chain fatty acid (C20- and C22-PUFA). Acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C20:4(n-6) acyl-CoA. -
Tissue specificity
Liver and testis. -
Sequence similarities
Belongs to the ELO family. -
Domain
The di-lysine motif may confer endoplasmic reticulum localization. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 54898 Human
- Entrez Gene: 54326 Mouse
- Entrez Gene: 498728 Rat
- Omim: 611814 Human
- SwissProt: Q9NXB9 Human
- SwissProt: Q9JLJ4 Mouse
- Unigene: 656436 Human
- Unigene: 2567 Mouse
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Alternative names
- 3 keto acyl CoA synthase ELOVL2 antibody
- 3-keto acyl-CoA synthase Elovl2 antibody
- Elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast) like 2 antibody
see all
Images
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Immunoprecipitation - Anti-ELOVL2 antibody [EPR11880] (ab176327)
Western blot analysis on immunoprecipitation pellet from mouse liver lysate labeling ELOVL2 using ab176327 at a 1/10 dilution.
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![Western blot - Anti-ELOVL2 antibody [EPR11880] (ab176327)](https://www.abcam.com/ps/products/176/ab176327/Images/ab176327-204124-anti-elovl2-antibody-epr11880-western-blot.jpg)
Western blot - Anti-ELOVL2 antibody [EPR11880] (ab176327)All lanes : Anti-ELOVL2 antibody [EPR11880] (ab176327) at 1/1000 dilution
Lane 1 : Rat liver lysate
Lane 2 : Mouse testis lysate
Lane 3 : Fetal liver lysate
Lane 4 : Mouse liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa -
![Anti-ELOVL2 antibody [EPR11880] (ab176327)](https://www.abcam.com/ps/products/176/ab176327/Images/ab176327-3-benefits-of-recombinant-antibodies.png)
Anti-ELOVL2 antibody [EPR11880] (ab176327)
Protocols
Datasheets and documents
References (4)
ab176327 has been referenced in 4 publications.
- Sarr O et al. Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency. J Nutr Biochem 63:140-149 (2019). PubMed: 30368227
- Ding Y et al. MYCN and PRC1 cooperatively repress docosahexaenoic acid synthesis in neuroblastoma via ELOVL2. J Exp Clin Cancer Res 38:498 (2019). PubMed: 31856871
- Song L et al. Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet. Sci Rep 7:41604 (2017). WB ; Rat . PubMed: 28169366
- Yan M et al. Identification of SPOP related metabolic pathways in prostate cancer. Oncotarget 8:103032-103046 (2017). WB . PubMed: 29262542
