Key features and details
- Rabbit polyclonal to ELOVL4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-ELOVL4 antibody
See all ELOVL4 primary antibodies
DescriptionRabbit polyclonal to ELOVL4
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Cynomolgus monkey, Rhesus monkey
- WB: HepG2 cell lysate; rat heart and liver tissue lysate. IHC: Human testis and small intestine tissue. ICC/IF: HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab224608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionCondensing enzyme that elongates saturated and monounsaturated very long chain fatty acids (VLCFAs). Elongates C24:0 and C26:0 acyl-CoAs. Seems to represent a photoreceptor-specific component of the fatty acid elongation system residing on the endoplasmic reticulum. May be implicated in docosahexaenoic acid (DHA) biosynthesis, which requires dietary consumption of the essential alpha-linolenic acid and a subsequent series of three elongation steps.
Tissue specificityExpressed in the retina and at much lower level in the brain. Ubiquitous, highest expression in thymus, followed by testis, small intestine, ovary, and prostate. Little or no espression in heart, lung, liver, or leukocates.
Involvement in diseaseDefects in ELOVL4 are the cause of Stargardt disease type 3 (STGD3) [MIM:600110]. STGD is one of the most frequent causes of macular degeneration in childhood. It is characterized by macular dystrophy with juvenile-onset, rapidly progressive course, alterations of the peripheral retina, and subretinal deposition of lipofuscin-like material. STGD3 inheritance is autosomal dominant.
Sequence similaritiesBelongs to the ELO family.
DomainThe di-lysine motif may confer endoplasmic reticulum localization.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 3-keto acyl-CoA synthase ELOVL4 antibody
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All lanes : Anti-ELOVL4 antibody (ab224608) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat liver lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Blocking: 5% milk (non-fat dry milk dissolved in PBST) incubate overnight under 4 oC.
Primary antibody incubation: Dilute primary antibody with 5% milk (non-fat dry milk dissolved in PBST), incubate overnight at 4oC.
Washing: PBST, 4 times, 10 minutes.
Secondary antibody incubation: Dilute secondary antibody at 1/10000 or 1/20000 with 5% milk (non-fat dry milk dissolved in PBST), incubate for 1 hour under 25oC.
Washing: PBST, 4 times, 10 minutes.
Paraffin-embedded human testis tissue stained for ELOVL4 using ab224608 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for ELOVL4 using ab224608 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ELOVL4 (green) using ab224608 at 1/100 dilution in ICC/IF.
Secondary antibody: Alexa Fluor® 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224608 has not yet been referenced specifically in any publications.