Overview

  • Product name

  • Description

    Rabbit polyclonal to ELOVL6/LCE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ELOVL6/LCE (C terminal).
    Database link: NP_076995
    (Peptide available as ab114079)

  • Positive control

    • Human brain tissue lysate.
  • General notes

     This product was previously labelled as ELOVL6

     

Properties

Applications

Our Abpromise guarantee covers the use of ab69857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).Can be blocked with ELOVL6/LCE peptide (ab114079).

Target

  • Function

    Condensing enzyme that catalyzes the synthesis of saturated and monounsaturated fatty acids. Highest activity toward C16:0 acyl-CoAs.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the ELO family.
  • Post-translational
    modifications

    N-Glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-keto acyl-CoA synthase ELOVL6 antibody
    • Elongation of very long chain fatty acids protein 6 antibody
    • ELOV6_HUMAN antibody
    • ELOVL family member 6, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3 like, yeast) antibody
    • ELOVL fatty acid elongase 6 antibody
    • ELOVL6 antibody
    • FACE antibody
    • FAE antibody
    • Fatty acid elongase 2 antibody
    • Fatty acyl CoA elongase antibody
    • Fatty acyl-CoA elongase antibody
    • FLJ23378 antibody
    • hELO2 antibody
    • LCE antibody
    • Long chain fatty acyl elongase antibody
    • Long-chain fatty-acyl elongase antibody
    • MGC5487 antibody
    • Myelin associated SUR4 protein antibody
    see all

Images

  • Lane 1 : Anti-ELOVL6/LCE antibody (ab69857) at 1 µg/ml
    Lane 2 : Anti-ELOVL6/LCE antibody (ab69857) at 2 µg/ml

    All lanes : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 31 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry of ELOVL6/LCE in human brain with ab69857 at 5 µg/ml.

  • ICC/IF image of ab69857 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69857, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of ELOVL6/LCE in Human Brain cells using ab69857 at 20 ug/ml.

References

This product has been referenced in:

  • Ducheix S  et al. Dietary oleic acid regulates hepatic lipogenesis through a liver X receptor-dependent signaling. PLoS One 12:e0181393 (2017). Read more (PubMed: 28732092) »
  • Shinohara S  et al. Ethanol extracts of chickpeas alter the total lipid content and expression levels of genes related to fatty acid metabolism in mouse 3T3-L1 adipocytes. Int J Mol Med 38:574-84 (2016). Read more (PubMed: 27353085) »
See all 5 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge replacement vial on the order *** which should arrive tomorrow.

Please keep me updated about the results with this new vial, and let me know if you have any questions or need anything else from me. Have a nice day.

Read More

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