Key features and details
- Rabbit polyclonal to ELOVL6/LCE
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ELOVL6/LCE antibody
See all ELOVL6/LCE primary antibodies
DescriptionRabbit polyclonal to ELOVL6/LCE
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human brain tissue lysate.
This product was previously labelled as ELOVL6
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab69857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).Can be blocked with ELOVL6/LCE peptide (ab114079).|
FunctionCondensing enzyme that catalyzes the synthesis of saturated and monounsaturated fatty acids. Highest activity toward C16:0 acyl-CoAs.
Sequence similaritiesBelongs to the ELO family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 3-keto acyl-CoA synthase ELOVL6 antibody
- Elongation of very long chain fatty acids protein 6 antibody
- ELOV6_HUMAN antibody
Lane 1 : Anti-ELOVL6/LCE antibody (ab69857) at 1 µg/ml
Lane 2 : Anti-ELOVL6/LCE antibody (ab69857) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 31 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Immunohistochemistry of ELOVL6/LCE in human brain with ab69857 at 5 µg/ml.
ICC/IF image of ab69857 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69857, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of ELOVL6/LCE in Human Brain cells using ab69857 at 20 ug/ml.
ab69857 has been referenced in 7 publications.
- Mika A et al. Exercise and Conjugated Linoleic Acid Supplementation Induce Changes in the Composition of Liver Fatty Acids. Front Physiol 10:602 (2019). PubMed: 31156468
- Li H et al. Decreased expression levels of ELOVL6 indicate poor prognosis in hepatocellular carcinoma. Oncol Lett 18:6214-6220 (2019). PubMed: 31788097
- Ducheix S et al. Dietary oleic acid regulates hepatic lipogenesis through a liver X receptor-dependent signaling. PLoS One 12:e0181393 (2017). PubMed: 28732092
- Shinohara S et al. Ethanol extracts of chickpeas alter the total lipid content and expression levels of genes related to fatty acid metabolism in mouse 3T3-L1 adipocytes. Int J Mol Med 38:574-84 (2016). PubMed: 27353085
- Marks KA et al. Hepatic and plasma sex differences in saturated and monounsaturated fatty acids are associated with differences in expression of elongase 6, but not stearoyl-CoA desaturase in Sprague-Dawley rats. Genes Nutr 8:317-27 (2013). WB ; Rat . PubMed: 23180365
- Chamulitrat W et al. Ursodeoxycholyl lysophosphatidylethanolamide inhibits lipoapoptosis by shifting fatty acid pools toward monosaturated and polyunsaturated fatty acids in mouse hepatocytes. Mol Pharmacol 84:696-709 (2013). Mouse . PubMed: 23974795
- Jump DB et al. Soraphen A, an inhibitor of acetyl CoA carboxylase activity, interferes with fatty acid elongation. Biochem Pharmacol 81:649-60 (2011). WB ; Human . PubMed: 21184748